The research for the therapy of myoma uteri and endometriosis using Gn-RH receptor
| Project/Area Number |
05671381
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagasaki University |
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Principal Investigator |
ISHIMARU Tadayuki Nagasaki University. Medicine professor, 医学部, 教授 (20039580)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Takanori Nagasaki University. Medicine Assistant, 医学部, 助手 (80200682)
MASUZAKI Hideaki Nagasaki University. Medicine Lecturer, 医学部, 講師 (00173740)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Gn-RH / Receptor / Autoradiography / Myoma Uteri / Endometriosis / GnRH analogue / 受容体 / - |
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| Research Abstract |
We exam ined whether gonadotrop in releasing horm one receptor (Gn-RH receptor) existed or did not exist in normal myometrium and myoma nodule using the auto radiography method. We used ^<125>I-Buserelin as Gn-RH analogue and used pituitary anterior lobe and hippocampus of rat as control samples. We recognized there were specific bindings of ^<125>I-Buserelin to pituitary anterior lobe and hippocampus of rat. However, the binding activities of ^<125>I-Buserelin to normal myometrium and myoma nodule were variable and we could not demonstrate their specific binding sites of ^<125>I-Buscrelin in those tissues. Although some incubation conditions, that were preincubation time and incubation temperature et al. were changed, we could not find the specific binding sites of ^<125>I-Buserelin in normal myometrium and myoma nodule. In conclusion, we could not demonstrate the existence of Gn-RH receptor in myometrium and myoma nodule.
In the next stage, using cell culture system we examined whether Gn-RHa had the direct effects to uterine myoma. 1x10^5 cells/dish of myoma and normal myometrium were cultured in the medium which contained several concentrations of Gn-RHa, and the number of cells/dish and the volume of protein/dish were analyzed every 2 days for 2 weeks. There were no significant differences in the number of cells/dish and the volume of protein/dish between the Gn-RHa contained group and the control group. Therefore, we guessed that Gn-RHa had no direct effect to the proliferative ability of myoma and normal myometrial cells.
In conclusion, it was guessed that the effect of Gn-RHa that reduced the volume of uterine myoma was not a direct effect of Gn-RHa through Gn-RH receptor but an indirect effect which was, for example, due to low estrogenic environment.
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Report
(4 results)
Research Products
(4 results)
