| Project/Area Number |
05671400
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Teikyo University School of Medicine |
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Principal Investigator |
OGINO Masahiro Teikyo University School of Medicine Assistant professor, 医学部, 助教授 (80107680)
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| Co-Investigator(Kenkyū-buntansha) |
KASHIMA Atsuko Teikyo University School of Medicine Assistant, 医学部, 助手 (00246050)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | endometrial cancer / c-erbB-2 / estorogen / cancer cell line / Ishikawa cell line / OMC-2 cel line / 子宮体癌 |
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| Research Abstract |
It is known that malignant transformation is a multistep process in which damage to genes normally involved in growth regulation culminates in tumor formation.
An attempt was made to elucidate the relationship between the estradiol and c-erbB-2 oncogene in the human endometrial cancer cell line in vitro. we examined two of celline ; Ishikasa cell line (Dr.Nishida : Tukuba Univ.) having estorogen reseptor positive (ER) and progesterone receptor positive (PR), and OMC-2 (Dr.Ueda : Osaka Med) having neiter ER nor PR.The proliferation of endometrial cancer cells of Ishikawa line was more stimulated after the 7 days than the control at concentraion 10 ^<-8>M estradiol. But such the proliferation phenomenon was not observed in OMC-2. Ishikawa cell line added estradiol at a concentration of 10^<-8>M was presented the c-erbB-2 mRNA expression, but Ishikawa cell line without estrdiol stimulation was slightly decreased after 14 days culture. OMC cell line was not presnted the c-erbB-2 mRNA expression in the both conditions. The c-erbB-2 mRNA expression can play an important role in the development of cell proliferation mediated throught the hormone receptor in the human endometrial cancer.
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