| Project/Area Number |
05671409
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
TOKI Naoyuki University of Occupational and Environmental Health, Obstet. Gynecol., Assistant, 医学部, 助手 (50237062)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUURA Yusuke University of Occupational and Environmental Health, Obstet. Gynecol., Assistant, 医学部, 助手 (90248584)
SUGIHARA Koichiro University of Occupational and Environmental Health, Obstet. Gynecol., Assistant, 医学部, 助手 (80248583)
KASHIMURA Masamichi University of Occupational and Environmental Health, Obstet. Gynecol., Professor, 医学部, 教授 (80117085)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Cervical Cancer / Angiogenesis / Molecular Pathology |
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| Research Abstract |
Angiogenesis, the induction of new capillaries and venules, has been highly associated with tumor growth. To examine whether tumor angiogenesis could serve as a prognostic factor in the uterine cervical carcinoma or not, we counted microvessels in the patient with squamous cell carcinoma of the uterine cervix. Sections of surgical specimen were stained with anti-Factor VIII related antigen antibody to identify all vessels. The microvessels were counted by light microscopy (per 200X field) in each tumor section with the highest population of microvessels. Microvessel count in the patients with squamous cell carcinoma was significantly increased compared with that in the control cases. There was no correlation between microvessel count and lymph node metastasis, tumor size, depth of stromal invasion, and prognosis. Microvessel count was significantly correlated with vascular space involvement (p<0.05).
Next, we have immunohistochemically examined the expression of platelet-derived endothelial-cell growth factor (PD-ECGF) in the uterine cervical squamous cell carcinoma. The expression of PD-ECGF,in the carcinoma cells and in the infiltrating macrophages, was not associated with the microvessel density assessed by immunostaining to Factor VIII related antigen. And there was no correlation between expression of PD-ECGF and PCNA (proliferating cell nuclear antigen) or p53 protein expression. It was difficult to utilize PD-ECGF expression as a prognostic factor.
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