PREVENTION OF HYPERSENSITIVITY ON RESPIRATORY TRACT BY SUPPRESSING CHEMOTACTIC ACIVITIES OF MAST CELLS
| Project/Area Number |
05671445
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | THE JIKEI UNIVERSITY SCHOLL OF MEDICINE |
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Principal Investigator |
IMI Toru THE JIKEI UNIVERSITY SCHOOL OF MEDICINE,OTORHINOLARYNGOLOGY,LECTURER, 医学部, 講師 (00130146)
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| Co-Investigator(Kenkyū-buntansha) |
SANEYOSHI Kensaku THE JIKEI UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (60246410)
NOHARA Osamu THE JIKEI UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (20237861)
KATAYAMA Noboru THE JIKEI UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (60260949)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Mast cell / Chemotaxis / IL-4 / Cytokine / Chemokine / Hypersensitivity / Mouse / Lymphocyte |
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| Research Abstract |
We obtained mouse cultured mast cell lines from bone marrow cells of BALB/c mice and used these lines for the experiments of mast cell chemotaxis. These lines showed several amount of histamine content and had several chemotactic activities with increasing maturation periods. The supernatant of T cell lines from mice which were immunized with cedar pollens secreted high amount of IL-4 and reacted as a chemotactant for cultured mast cells. when cultured mast cells were activated, they increased chemotactic activities and producted IL-4 10 times higher than T cell lines. In contrast, the use of steroid and treatment with heat stress suppressed chemotactic activities of mast cells. These results indicated that IL-4 production may cause local hypersensitivity during allergic reaction and the therapy should be useful to prevent it.
To know the activity as a chemotactant of T cell products in human, we stimulated peripheral T cells from the patients suffering from cedar pollinosis. The T cells from patients proliferated markedly with purified cedar pollens and increased to pruduce IL-4 but decreased to secrete IL-gamma. The immunotherapy suppressed the proliferation of T cells and production of IL-4. The culture of mast cell lines were not established from umbilical cord blood.
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Report
(4 results)
Research Products
(21 results)
