| Project/Area Number |
05671496
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児外科
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
ZAIZEN Yoshio Kyusyu University, Faculty of Medicine, Department of Pediatric Surgery, Assistant Professor., 医学部, 講師 (50221289)
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| Co-Investigator(Kenkyū-buntansha) |
SUITA Sachiyo Kyusyu University, Faculty of Medicine, Department of Pediatric Surgery, Profess, 医学部, 教授 (30038856)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Neuroblastoma / Invasion / Insulin-like growth factor II / Retinoic acid / N-myc / Cellular motility / 細胞骨格蛋白 |
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| Research Abstract |
Insulin-like growth factor II (IGF-II) is implicated in the development of the vertebrate neural circuitry, and increases neurite growth in vitro and in vivo. We examined the relationship of IGF-II expression to the in vitro differentiation induced by retinoic acid (RA). We find that RA stimulates an increase in IGF-II messenger RNA (mRNA) in the SK-N-SH (SH) neuroblastoma cell kine. An increase of IGF-II mRNA is detected within 12 h of treatment and precedes morphological differentiation. A RA dose response test indicates that an increase in IGF-II mRNA occurs within 2 days in SH cells treated with doses of RA from 10^<-8> to 10^<-5> M.We suggest that IGF-II expression may be RA in vitro and may lead to neuroblastoma differentiation.
The invasiveness of IMR-32 and GOTO,of which amplification of N-myc gene was 15 and 12 copies respectively, was 9.1 times and 8.8 times higher than that of SK-N-SH without N-myc amplification. Using a video-imaging analysis, we directly measured the motility of neuroblastoma cells on culture flask. These trace images clearly show that the motility of IMR-32 and GOTO is 2.3-1.7 times higher than that of SK-N-SH which had extremely low invasiveness and motility. To clarify the effect of reduction in N-myc gene expression on invasive capacity and cellular motility, GOTO was treated 10^<-5> MRA for 72 hours before examination. RA treatment markedly reduced the N-myc gene expression in approximately one quarter of the control and apparently decreased invasive capacity of GOTO as well as cellular motility which declined about one fifth of untreated GOTO.
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