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Study of Entamoeba gingivalis in dental plaque and its association with periodontal diseases.

Research Project

Project/Area Number 05671515
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionShowa University

Principal Investigator

YAMAMOTO Ayako  Showa University, school of Dentistry, Associated Prof., 歯学部, 助教授 (20085773)

Co-Investigator(Kenkyū-buntansha) GOTO Nobuichi  Showa University, school of Dentistry, Prof., 歯学部, 教授 (10077175)
菊田 典子  昭和大学, 歯学部, 助手 (50255874)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsEntamoeba gingivalis / Oral amoeba / SrRNA / Species-specific DNA probe / Phylogenetic analysis / Protozoan / Phylogenetic analysis / ribosomal DNA probe / Oligomucleotide probe / digoxigenin labeled probe / oral amoeba / in situ hybridization
Research Abstract

The small subunit ribosomal RNA (SrRNA) gene of Entamoeba gingivalis was amplified by PCR and the product of 1.9-kb long was cloned into a plasmid vector pUC18. Four clones were isolated and sequenced. The insert DNAs were 1918-to 1921-bp long and A+T rich (65.5%). The four SrRNA sequences of E.gingivalis were aligned with those of nine related protozoans searching for E.gingivalis-specific sequence. A sequence of 28 oligonucleotides was chosen, chemically synthesized, and labeled with digoxigenin for a DNA probe. The probe thus constructed was shown to hybridize only with either the SrRNA-coding DNAs or the cells of the two E.gingivalis strains and not with those of other protozoans and oral fungi tested. A representative SrRNA-sequence was analyzed and a phylogenetic tree was constructed by neighbor-joining (NJ) method. Among the protists examined, E.gingivalis was placed next to Entamoeba histolytica as expected from the traditional taxonomy.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Ayako Yamamoto et.al: "Nucleotide sequence of the SrRNA gene of Entamoeba gingivalis:Appliortions for construction of a species-specific DNA probe and phylogenetic analysis" Microbiology and Immunology. 39. 185-192 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ayako Yamamoto: "Nucleotide sequence of the SrRNA gene of Entamoeba gingivalis : Applications for construction of a species-specific DNA probe and phylogenetic analysis." Microbiology and Immunology. 39. 185-192 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ayako Yamamoto: "Nucleotide sequence of the SrRNA gene of Entamoeba gingivalis:Applications for construction of a species-specific DNA probe and phylogenetic analysis" Microbiology and Immunology. 39. 185-192 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 山本,綾子: "Entamoeba gingivalisの16S rRNA遺伝子の塩基配列" 日本細菌学雑誌. 48. 147 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 山本,綾子: "歯肉アメーバ特異的検出用DNAプローブの作成と特異性の検討" 日本細菌学雑誌. 49. 199 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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