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ENHANCEMENT OF GROWTH OF PERIODONTOPATHIC BACTERIA BY PRO-INFLAMMATORY CYTOKINES

Research Project

Project/Area Number 05671520
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionNIHON UNIVERSITY

Principal Investigator

OCHIAI Tomoko  NIHON UNIVERSITY,SCHOOL OF DENTISTRY AT MATSUDO,INSTRUCTER, 松戸歯学部, 助手 (20130594)

Co-Investigator(Kenkyū-buntansha) OCHIAI Kuniyasu  NIHON UNIVERSITY,SCHOOL OF DENTISTRY AT MATSUDO,ASSISTANT PROFESSOR, 松戸歯学部, 講師 (50095444)
FUKUSHIMA Kazuo  NIHON UNIVERSITY,SCHOOL OF DENTISTRY AT MATSUDO,PROFESSOR, 松戸歯学部, 教授 (20009327)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsGrowth / Periodontopathic bacteria / IL-1alpha / IL-1beta / IL-6 / 歯周病原性 細菌 / IL-1beta / IL-1alpha
Research Abstract

(1) Effect of cytokines on bacteria growth : we investigated the effect of IL-alpha, beta and IL-6 on bacterial growth. The growth-promoting effect of IL-1alpha, beta over control (no IL-1) was observed during the log phase of Actinobacillus growth (9h : 1.6-1.9 times, 18th : 1.5-1.7 times) . IL-1 alpha at 1 to 100 ng/ml enhanced Actinobacillus growth after 9h incubation compared to control. IL-1beta at 10 and 100 ng/ml promoted Actinobacillus growth. IL-1 alpha, beta also had a smaller effect on Capnocytophaga and Fusobacterium growth.
(2) Binding of ^<125>I-IL-1alpha, beta and IL-6 to bacteria : Actinobacillus showed a high level of uptake of the radiolabeled IL-1alpha, beta and IL-6, While Capnocytophaga and Fusobacterium bound a very low amount of ^<125>I-IL-1alpha and IL-6. Other strains including Porphyromonas, Prevotella and Eikenella did not bind to cytokines.
(3) Homology search with human cytokine receptor gene : When we examined bacteria for gene expression of cytokine receptor, expression of IL-IR (I) and IL-IR (II) was only detected in Actinobacillus, not other bacteria such as Porphyromonas, Prevotella, Fusobacterium and Capnocytophaga. On the other hand, IL-6R expression was not detected in all the strains used.
(4) Autocrine mechanism against bacteria : We examined IL-Ialpha, beta and IL-6 cytokine activity in bacterial culture supernatants. IL-1alpha activity was detected slightly in Fusobacterium and Capnocytophaga, but was not significant. Trace amounts of IL-I beta ware detected in all the strains used, and especially higher level of IL-I beta could be detected in Actinobacillus. IL-6 was not produced in all the strains.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

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