| Project/Area Number |
05671608
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | KANAGAWA DENTAL COLLEGE |
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Principal Investigator |
DEGUCHI Shinji KANAGAWA DENTAL COLLEGE,PERIODONTOLOGY,ASSOCIATED PROFESSOR., 歯学部, 助教授 (60121018)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASE Toshio KANAGAWA DENTAL COLLAGE,ORAL BIOCHEMISTRY,ASSOCIATED PROFESSOR., 歯学部, 助教授 (30084784)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | HUMAN PERIODONTIUM DERIVED CELLS / CHEMOTAXIS / CONDITIONED MEDIUM / CELL ATTACHMENT AND SPREADING / 歯周組織由来細胞 / 歯根膜由来線維芽細胞 / Conditioned mediom |
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| Research Abstract |
We have shown that the conditioned medium (HPLF-CM) enhanced attachment and spreading of human periodontal ligament fibroblast (HPLF). The present study was attempted to evaluate the chemotaxis, attachment and spreading of human periodontal tissue derived cells (HPLF,HGF,HABC,HGE) and human peripheral blood neutrophill (PMN). We found that HPLF-CM not stimulated only the chemotactic migration but also the attachment and spreading of all of used human periodontal tissue derived cells. HPLF was stimulated the chemotactic migration a wide range of HPLF-CM concentration (35 mug/ml-70 ng/ml). HGF was also indicated high chemotactic activity at a molecular weight of approximately 70 kDa of HPLF-CM as determined by fractioned in a PO-60k column. HABC was stimulated chemotactic activity with 8.7 mug-70 ng/ml HPLF-CM,but not with 35 mug/ml HPLF-CM.However, 350,175 ng/ml HPLF-CM were not enhanced the migration of PMN compared with 10^<-7> M FMLP.Hence we conclude that HPLF-CM contains the cell attachment, spreading and chemotactic factors, which may contribute to the development and regeneration of connective tissue attachment in vivo.
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