| Project/Area Number |
05671689
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | TOKYO DENTAL COLLEGE |
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Principal Investigator |
YAMA Misturu TOKYO DENTAL COLLEGE,Ichikawa Gneral Hospital, Dept of Surgery, Assitant, 歯学部, 助手 (60230299)
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Yasuhiro Tokyo Dental College, Ichikawa Gneral Hospital, Dept of Surgery, Assitant, 歯学部, 助手 (70246354)
SHIBAHARA Takahiko TOKYO DENTAL COLLEGE,Ichikawa Gneral Hospital, Dept of Surgery, Assitant Profess, 歯学部, 講師 (50178919)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | bone-inducing substance / tooth / westerm blotting / nitrosellulose membrane / polyviailideme difluoride membrane / bone-induction / amino acid sequence / osteocalcin / ポリビニリデンジフロライド膜 / 軟骨性骨化 |
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| Research Abstract |
A bone-inducing substance was extracted from demineralized and defatted bovine tooth matrix by mean of 6M guanidine hydrochloride solution. This study attempt to analyze the bone induction properties of this substance, the associated bone-inducing process, and to purify the substance to derive the fraction with bone induction properties.
Results
1. The bone-inducing substance was extracted from demineralized and defatted bovine tooth matrix at the rate of 0.48%.
2. After implanting this bone-inducing substance femoral ; muscle of mice, typical enchondral ossification occured.
3. After this bone-inducing substance was purified by gel-filtration, four fractions were obtained.Only the second fraction demonstrated bone-inducing activity.
4. In SDS-PAGE,the second fraction had three major bands, with molecular weight of about 28000,20000 and 14000
5. After these major bands were electroblotted from SDS-PAGE on to nitrocellulose membrane, each of about 28000 demonstrated bone induction.
6. The component demonstrated bone inducing activity is a unique protein. The same component extracted from bovine bone matrix was showed that the first 15 amino acid were the same as the bovine osteocalcin in NH2-terminal region. It suggest that amino acid sequence of the bone inducing substance extracted from human tooth matrix are the same as the human osteocalcin in NH2-terminal region.
7. Bone-inducing substance contained in bone and tooth is concerned in producing and remodeling of bone.
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