| Project/Area Number |
05671788
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | HEALTH SCIENCES UNIVERSITY OF HOKKAIDO |
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Principal Investigator |
TOHMA Masahiko HEALTH SCIENCES UNIVERSITY OF HOKKAIDO,FAC.PHARMACEUTICAL SCIENCES,PROFESSOR, 薬学部, 教授 (30001043)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Akihiko KURUME UNIVERSITY SCHOOL OF MEDICINE,INSTRUCTOR, 医学部, 助手 (00211201)
YOSHIMURA Teruki HEALTH SCIENCES UNIVERSITY OF HOKKAIDO,FAC.PHARM.SCIENCES,INSTRUCTOR, 薬学部, 助手 (60220737)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | FETAL BILE ACID / BILE ACID PROFILE / NEONATAL LIVER DISTEASE / GAS CHROMATOGRAPHY-MASS SPECTROMETRY / BILE ACID ENZYMATIC BIOSYNTHESIS / HYPERTYROSINEMIA CHOLESTASIS / 3-0X0 UNSATURATED BILE ACID / RAT LIVER MICROSOME / 未熟児 / 高速液体クロマトグラフィー / 胎児性胆汁酸合成 / カニクイザル肝ミクロゾーム / ガスクロマトグラフイー質量分析 / 高速液体クロマトグラフイー |
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| Research Abstract |
The metabolism of bile acids in fetus and neonate is thought to be different from that in adults. A highly sensitive analytical metod of bile acids by GC-MS has been required to elucidate the composition of fetal bile acids in serum for the diagnosis of neonatal hepatobiliary diseases.
(1) Symthesis of 4beta- and 6alpha-hydroxy bile acids were efficiently carried out for the identification of these acid in urine and serum from neonates by the comparison with the standards.
(2) A higly sensitive GC-MS determination of the fetal bile acid in neonatal serum and dry blood spot disc has been developed by capillary GC-MS using selected ion monitoring with negative ion chemical ionization.
(3) The fetal bile acids are usualy biosynthesized thrugh the hydroxylation at 1beta- or 6alpha-psition of the correspnding usual bile acids. The 1beta-hydroxylated C_<27>-bile acid were detected in the incubation mixture of the C_<27>-intermediates with rat liver microsome as a new biosynthetic route.
(4) Diagnostic analysis of neonatal liver diseases was accomplished by GC-MS.The 3-oxo unsaturated bile acids were identified and determined in the urine of patients with sevear cholestasis from hypertyrosinemia.
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