| Project/Area Number |
05671821
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
MORIYAMA Yoshinori Osaka University Institute of Scientific and Industrial Research Research Associate, 産業化学研究所, 助手 (10150658)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Glutamate / Synaptic vesicle / Transmitter / Vacuolar ATPase / Newron / Transporter / 輸送系 / 液胞型ATPase |
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| Research Abstract |
Synaptic vesicles are storage subcelluar organelles for neurotransmitters in presynaptic nerve terminals and possess at least four different kinds of transporters for neurotransmitters : One of them, a glutamate transporter is very important transporter, because glutamate is an exicitatory amino acid transmitter. So far little is known about the glutamate transporter. In this study I investigated several aspects of the transporter : (1) purification, (2) specific inhibitors, (3) biochemical study on vacuolar ATPase as a primary pump for glutamate transport. During the past two years, I succeeded at least in part in carrying out the above projects : I established the procedure for solubilization, reconstitution and partial purification of glutamate transporter. I found that dibezylglutamate as a potent competitive inhibitor for the transporter. I determinied that there are binding sites for omeprazole and quinacrine mustard in subunit A of chromaffin granules vacuolar ATPase. By cross-linking technique, I obtained several useful information on subunit/subunit interaction of vacuolar ATPases. These results are important for understanding entire feature of vesicular glutamate transporter.
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