| Project/Area Number |
05671837
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | University of Shizuoka |
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Principal Investigator |
TAKEDA Atushi University of Shizuoka School of Pharmacertical Sciences Assistant Professor, 薬学部, 講師 (90145714)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Shoji University of Shizuoka School of Pharmacertical Sciences Professor, 薬学部, 教授 (40046256)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Zinc / Manganese / Brain / Blood-cerebrospinal fluid-barrier / Choroid plexus / Brain-imaging agent / 脳機能解析 / 神経調節因子 |
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| Research Abstract |
The brains of rats injected intravenously (i.v.) with ^<65>ZnCl_2 or ^<54>MnCl_2 were subjected to high reso lution autoradiography.^<65>Zn and ^<54>Mn were largely concentrated in choroid plexus 1 h after injection and then gradually decreased, with increases in other brain regions, suggesting that both metals were taken up gradually into brain mainly via cerebrospinal fluid (CSF) in the choroid plexus.
To understand the contribution of Blood-CSF-barrier to the uptake of both metals, the brains of rats, after ^<65>ZnCl_2 or ^<54>MnCl_2 injection into the lateral ventricle, were subjected to the autoradiography.The autoradiogram obtained revealed that ^<65>Zn and ^<54>Mn were transferred freely through the cerebrospinal and extracellular fluid compartments ; both tracers appeared in all the ventricles 1 h after injection.At 6 days after injection.^<65>Zn was concentrated in the hippocamus and hypothalamic unclei but not in the cerebral cortex and midbrain of the ipsilateral hemisphere, with a low uptake into contralateral hemisphere except for the hypothalamic nuclei.^<54>Mn was concentrated in some brainstem nuclei to about the same extent in both hemisphere.These results suggest that both metals were taken up gradually into brain mainly via CSF.Relatively high uptake of ^<65>Zn into the cerebral cortex and midbrain after i.v.injection suggests uptake in those regions in through two blood/brain barriers.
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