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OXIDATIVE DETERIORATION OF SYNAPSE DURING AGING AND ITS PROTECTION

Research Project

Project/Area Number 05671863
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionTOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY

Principal Investigator

URANO Shiro  TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY BIOCHEMISTRY AND ISOTOPES SENIOR RESEARCH SCIENTIST, 老化科学技術研究系・アイソトープ部門, 主査 (20073009)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsSynapse / Oxidative injury / Aging / Antioxidation / Vitamin E / Antioxidative enzyme / フリーラジカル / 過酸化脂質
Research Abstract

In order to verify whether active oxygen species actually attack nervous systems and make the oxidative injury, resulting in the deterioration of the physiological functions in nerve terminals, and whether the deterioration is correlated with brain aging, we investigated the effect of peroxide on the functions of nerve terminal in rats which were exposed by 100% oxygen atmosphere. Results obtained as follows :
Through oxidative stress, the morphological changes ; i.e.swelling of astrocytes around the vessels, deformation of nuclei of nerve cells, mitochondrial swelling, and the abnormal accumulation of synaptic vesicles in the terminals were observed. Release of acetylcholine from the terminal through the stimulation of veratridine was significantly decreased. The membrane permeability to glucose was increased and the fluidity was decreased by oxygen exposure and with aging. The content of lipid peroxide in the membranes was higher than that of the unexposed rats, and the content of unsaturated fatty acids, especially arachidonic acid and docosahexaenoic acid was significantly decreased. Although catalase and glutathione peroxidase activities were increased, Superoxide dismutase activity was decreased by oxygen exposure and with aging.
On the basis of these findings, it was thought that active oxygen species may attack the nerve terminals, and hence, peroxidize the synaptic membranes, resulting in deterioration of synaptic membranes.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 浦野四郎: "ラット,シナプスの酸化損傷と老化" ビタミンE研究の進歩. IV. 85-89 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 浦野四郎: "生体膜損傷と抗酸化" Therapeutic Research. 15(8). 81-85 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shiro Urano: "Oxidative damage of synaptic membranes in rats during aging" Biomed. Gerontol.18(2). 378-379 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 浦野四郎: "老化のメカニズム" 沖縄医学界雑誌. 32(2). 126-131 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 浦野四郎: "老化と環境因子" 松尾光芳編 学会出版センター, 224 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shiro, Urano: "Oxidative Injury of Synapse and Aging in Rats" Progress in Vitamin research. IV. 85-89 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shiro, Urano: "Oxidative Injury of Membranes and antioxidation" Therapeutic Research. 15. 81-85 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shiro, Urano: "Oxidative Damage of Synaptic membranes in rat during Aging" Biomed.Gerontol.18. 378-379 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shiro, Urano: "Mechanism of Aging" Okinawa Medical Journal. 32. 126-131 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shiro, Urano: "Aging and Circumstantial". ed.by Mitsuyoshi, Matsuo, Gakkai Syppan Center, Tokyo, 224 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 浦野 四郎: "生体膜損傷と抗酸化" Therapeutic Research. 15(8). 81-85 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shiro Urano: "Oxidative damaqe of synaptic membranes in rats during aging" Biomed Gerontol.18(2). 378-379 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 浦野 四郎: "老化のメカニズム" 沖縄医学界雑誌. 32(2). 126-131 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 浦野 四郎: "老化と環境因子" 松尾 光芳編 学会出版センター, 224 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 浦野 四郎: "ラット,シナプスの酸化損傷と老化" ビタミンE研究の進歩. IV. 85-89 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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