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Investigation of Steroid Sulfate Conjugates in Neonatal Serum-Elucidation of the Mechanism of the Biosynthesis of the Steroids and Its Application for Pathological Analysis.

Research Project

Project/Area Number 05671935
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionKobe Pharmaceutical University

Principal Investigator

WATANABE Fukuko  Kobe Pharmaceutical University, Faculty of Pharmacy, Professor, 薬学部, 教授 (10068322)

Co-Investigator(Kenkyū-buntansha) SUMINOE Akemi  Kobe Pharmaceutical University, Faculty of Pharmacy, Instructor, 薬学部, 助手 (40258124)
TAGAWA Noriko  Kobe Pharmaceutical University, Faculty of Pharmacy, Instructor, 薬学部, 助手 (00206905)
KOBAYASHI Yoshiharu  Kobe Pharmaceutical University, Faculty of Pharmacy, Associate Professor, 薬学部, 助教授 (20133647)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsAdrenal steroid / Sulfate conjugate / 16-Dehydropregnenolone 3-sulfate / 16-Dehydropregnenolone 3-sulfate / 16‐Dehydropregnenolne
Research Abstract

The present study was designed to investigate the conjugation form of 16-dehydropregnenolone (16-DHP) which had been identified in the serum of neonates in our laboratory and its metabolic site.
Free steroids were washed out from neonatal serum by CH_2Cl_2 treatment. The residue which may contain conjugated steroids was extracted with MeOH.The MeOH extracts were applied to HPLC.The fraction, in which authentic 16-DHP 3-sulfate was eluted, was collected. This fraction was treated with arylsulfatase. Liberated free steroids was analyzed by GC/MS and GC/FT-IR.All of the data obtained was identical with those of authentic 16-DHP.These results showed that 16-DHP is conjugated with sulfuric acid.
To investigate if 16-DHP 3-sulfate is synthesized in fetal adrenal, correlation of serum levels of 16-DHP 3-sulfate and gestational age was observed. As a result, significant negative correlation was observed between these two parameters. These results suggest that 16-DHP 3-sulfate is synthesized in fetal adrenal.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Yoshiharu Kobayashi: "Studies on Steroids in Fetuses and Neonates:Identification of 16-Dehydropregnenolone in the Circulation of Pre-term Neonates" Biol.Pharm.Bull.17. 1501-1504 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshiharu Kobayashi et al.: "Studies on steroids in fetuses and neonates : Identification of 16-dehydropregnenolne in the circulation of pre-term neonates" Biol.Pharm.Bull. 17. 1501-1504 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshiharu Kobayashi: "Studies on Steroids in Fetuses and Neonates:Identification of 16-Dehydropregnenolone in the Circulation of Pre-term Neonates" Biol.Pharm.Bull.

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshiharu Kobayashi: "Characteristics of Mass Fragmentation of Steroids by Atmospheric Pressure Chemical Ionization‐Mass Spectrometry" Biol.Pharm.Bull. 16. 1175-1178 (1933)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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