| Project/Area Number |
05680084
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
体育学
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
FUJITSUKA Noriaki Nagoya Institute of Technology, Department of Engineering, Professor, 工学部, 教授 (60101268)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKAMI Keisuke Nagoya University, Collage of Medical Technology, Assistant, 医療技術短期大学部, 助手 (60195047)
MURAKAMI Taro Nagoya Institute of Technology, Department of Engineering, Assistant, 工学部, 助手 (10252305)
YOSHIMURA Atsushi Nagoya Institute of Technology, Department of Engineering, Assistant Professor, 工学部, 助教授 (70159234)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | neuro-degeneration / rat hippocampus / swim training / spatial cognitive test / 神経細胞変性 / 細胞内Ca^<2+> / エネル-分散型X線分析 / トレーニング / 低酸素 / 低グルコース / マイクロダイアリシス / X線解析 / ラット海馬 / 持久的水泳運動 |
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| Research Abstract |
To study the effects of physical stress on the rat brain, four experiments were took-place in adults female and male rats. The title of them were as follows ; 1.Degeneration of rat hippocampal neurons in response to swim training, 2.Difference between left and right hemispheres of rat hippocampus in relation to exercise-induced neuro-degeneration, 3.Leaming and memory abilities of rats subjected to endurance swim training for 6 weeks, and 4. Energy dispersive X-ray analysis of Intracellular Ca^<2+> in the rat hippocampal CA3 subfield after endurance swim.
These experiments revealed that the exercise stress such as endurance swimming induced the serious neuronal damages and functional deterioration. In detail, Numerous dark neurons histochemically stained were found in the subiculum, CA3 subfield and dentate gyrus of rat hippoampus subjected to 2-3 h swim training for 6-12 weeks. The cell density was significantly decreased in the CA3 and subiculum after 12-week training. The profiles of darkly stained neurons were extremely similar to the ischemic and/or hypoglycemic neuro-degenerations previously reported. Also, the intensely degenerated neurons were observed in the amygdaroid, parietal cortex area 1, and piriform cortex of rat brain after 12-week swim training. In the hipocampus, the degenerated neurons induced by swim training were frequently found in the right rather than in the left hemisphere. Furthermore, Energy dispersive X-ray analysis confirmed that a bout of 2-h swimming increased the extracellular Ca^<2+> concentration of CA3 pyramidal neurons. Subjected to a spatial cognitive test, the learning ability of rats with exercise induced neuro-degeneration mentioned above was inferior to that of normal ones.
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