| Project/Area Number |
05680482
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
環境保全
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OMORI Toshio The Univ.of Tokyo, Bitech.Res.Center, Prof., 生物生産工学研究センター, 教授 (20011984)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | dibenzofuran / dioxin / meta ring-fission enzyme / fluorene / microbial degradation / シベンゾフラン / フレオレン / ジベンゾパラダイオキシン / ジベンゾチオフェレ |
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| Research Abstract |
To elucidate the genes of the enzymes including initial dioxygenases involved in the degradation pathways of dioxin and dibenzofuran by strain DBF63, the cloning of the genes and sequencing of the isolated genes were carried out.
One clone (pLM1) specifing meta cleavage enzyme has been isolated. Another gene for meta cleavage enzyme was also found out. The isolated gene for meta cleavage enzyme has Sph1 site. Restriction enzyme Sph1 was used to obtain another gene by shot gun cloning with use of host vector system of E.coli MV1184 and pUC119. An active tageted clone was detected by spraying the solution mixture of catechol, 3-methylcatechol, 4-methylcatechol and 3-phenylcatechol which are analogus compouds of substrate 2,2', 3-trihydroxybiphenyl to the colonies of transformants.
The newly obtained plasmid pKN1 has completely different restriction enzyme site with the other clone which has activity of meta ring-fission enzyme.
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