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Concurrent regulation ofcholesterol sulfotransferase and sulfatase

Research Project

Project/Area Number 05680519
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

IWAMORI Masao  University of Tokyo, Associate Professor, 医学部(医), 助教授 (90110022)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordscholesterol sulfate / sulfotransferase / sulfatase / transglutaminase / uterine endometrium / epithelial cells / skin / 皮膚 / スルファターゼ / 活性化蛋白質 / ステロイド / 受容体
Research Abstract

Cholesterol sulfatein the endometrium of rabbit uterus was increasedin the concentration per gram dry weight by pregnancy, and the concentration in the pregnant endometrium was 140-fold than that in the nonpregnant endometrium. In the endometrium of pregnant rabbit, one tenth of the total cholesterol was sulfated at the maxmum level and both of cholesterol sulfotransferase and cholesterol sulfate sulfatase were simultaneously regulated to elevate the concentration of cholesterol sulfate. Synthesis of cholesterol sulfate was found to occure in the epithelial cells of uterine endometrium, but not in the stromal cells. Concerning on the mechanism of induction of cholesterol sulfate in the pregnant endometrium, the cell density was found to be important. A stimulation with progesterone to the sparce culture of the endometrial cells was unable to induce cholesterol sulfate, but activation of cholesterol sulfotransferase occurred to the confluent culture with progesterone, indicating that responsiveness of the endometrial cells to steroid hormones requires for the cells to differentiate in the sufficient stage. This findings coincided that the sulfotransferase appeared in the endometrium of rabbits 4 days after administration of gonadtropin to secrete progesterone, though the endometrium initiated the proliferation. the same activation was observed in The confluent stage of keratinocytes. On the other hand, cholesterol sulfate was found tobe selectively distributed in the epithelial cells in the digestive organs and kidney. Although the spspecific activities of cholesterol sulfotransferase and cholesterol sulfate sulfatase and the concentration of metabolic precurson cholesterol were significantly high in liver, a programd expression of cholesterol sulfate and transglutaminase was observed during the development of skin. In fact, cholesterol sulfate characteristically activated protein kinase n and was related to the induction of transglutaminase.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Iwamori M, Tayama N, Nomura Y, Nagai Y: "Hormone-dependent enhancement in binding of oto-and nephrotoxic amnnoglycoside antibiotics" Acta Otolaryngology. 514. 117-121 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Momoeda M, Cui Y, Sawada Taketani Y, Iwamori M: "Pseudopregnancy dependent accumilation of cholesterol sulfate due to up-requlation of chcolesterol sulfotransferase" Journal of Boochemistry. 116. 657-662 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noguchi M, Suping Z, Iwamori M, Okumura K: "Unique T cell differentiation markers.Gangliosides with cholera toxin recepter activity on murine fetal thymocytes" Cellular Immunology. 156. 420-433 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kagehara M, Tachi M, Harii K, Iwamori M: "Programmed expression of cholesterol sulfotransferase and trans θ glutaminase during epidermal differentiation of murise skin" Biochimica et Biophysica Acta. 1215. 183-189 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Momoeda M, Momoeda K, Taketani Y, Iwamori M: "Characteristic expression of GDla ganglioside during lactation of murine mammary gland" Biochimica et Biophysica Acta. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kitano Y. Takaoka T, Harii K, Iwamori M: "Enhancement of adhesive property of epithelial cell line Mm2T by culture in the presence of methylated vitamin B12" Cell Structure and Function. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iwamori M,Tayama N,Nomura Y,Nagai Y: "Hormonedependent enhancement in binding of oto- and nehprotoxic aminoglycoside antibiotics" Acta Otolaryngology. 514. 117-121 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Momoeda M,Cui Y,Sawada Y,Taketani Y,Iwamori: "Pseudopregnancy dependent accumulation of cholesterol sulfate dure to up-regulation of cholesterol sulfate sulfatase in rabbit uterine endometrium." J.Biochem.116. 657-662 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Noguchi M,Suping Z,Iwamori M,Okumura K: "Unique T cell differentiation markers. gangliosides with cholera toxin receptor activity on murine fetal thymocytes." Cell Immunol.156. 420-433 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kagehara M,Tachi M,Harii K,Iwamori M: "Programd expression of cholesterol sulfotransferasse and transglutaminase during epidermal differentiation of murine skin." Biochim.Biophys.Acta. 1215. 183-189 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Momoeda M,Momoeda K,Takatani Y,Iwamori M: "Characteristic expression of GDlalpha gangliosides during lactation of murine mammary gland." Biochim.Biophys.Acta. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kitano Y,Takaoka T,Harii K,Iwamori M: "Enhance ment of adhesive property of epithelial cell line Mm2T by culture in the presence of methy lated vitamin B12." Cell Struct.Func.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iwamori M,Tayama N,Nomura Y,Nagai Y: "Hormone-dependent enhancement in binding of oto-and nephrotoxic aminoglycoside antibiotics" Acta Otolaryngology. 514. 117-121 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Momoeda M,Cui Y,Sawada,Taketani Y,Iwamori M: "Pseudopregnancy dependent accumulaton of cholesterol sulfate due to up-requlation of chcolesterol sulfotransferase" Journal of Biochemistry. 116. 657-662 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Noguchi M,Suping Z,Iwamori M,Okumura K: "Unique T cell differentiation markers.Gangliosides with cholera toxin receptor activity on murine fetal thymocytes" Cellular Immunology. 156. 420-433 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kagehara M,Tachi M,Harii K,Iwamori M: "Programmed expression of cholesterol sulfotransferase and transθ glutaminase during epidermal differentiation of murine skin" Biochimica et Biophysica Acta. 1215. 183-189 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Momoeda M,Momoeda K,Taketani Y,Iwamori M: "Characteristic expression of GDla ganglioside during lactation of murine mammary gland" Biochimica et Biophysica Acta. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kitano Y,Takaoka T,Harii K,Iwamori M: "Enhancement of adhesive property of epithelial cell line Mm2T by culture in the presence of methylated vitamin B12" Cell Structure and Fumction. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Cui,Y.,Noguchi,H.,Nozawa,S.and Iwamori,M.: "Human cervical epidermal-carcinoma associated intracellular localization of glycosphingolipid with blood group type 3 chain" Japanese Journal of Cancer Research. 84. 664-672 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Natomi,H.,Saitoh,T.,Sugano,K.and Iwamori,M.: "Systematic analysis of glycosphingolipids in the human gastro-intestinal tract:Enrichment of sulfatides with hydroxylated FA" Lipids. 28. 737-742 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takamatsu,K.,Kamei,K.,Kubushiro,K.,Iwamori,M.: "Luteal phase-characteristic induction of 13S03-Lacer in human cervical epithelia and uterine endometria" Biochim.Biophys.et Acta. 1170. 232-236 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Iwamori,M.,Hirayama,K.,Momoeda,K.,Hanaoka,K.: "Glycosphingolipids characteristic for fetal human brain just before myelin formation" Neurochemical Research. 18. 842 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Mikami,M.,Iwamori,M.,Nozawa,S.: "Characteristic alteration in the concentration of paragloboside in villi of human placenta during the gestational period" Placenta. 14. 407-416 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Iwamori,M.,Kiguchi,K.,Nozawa,S.,Hirohashi,S.: "Compensatory occurrence of fucosylGMl anf human fetal antigen,Lc4Cer in small cell carcinoma of human lung" Cancer Research. 53. 5903-5907 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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