| Project/Area Number |
05680588
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WATANABE Yoshonori The University of Tokyo, Graduate School of Science Research, associate, 大学院・理学系研究科, 助手 (20212326)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Masayuki The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (40114706)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | meiosis / RNA / RNA recognition motif / premeiotic DNA synthesis / first meiotic division / fission yeast / 減数分裂前DNA合成 / RNA結合タンパク質 / キナーゼ |
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| Research Abstract |
The molecular controls over meiosis are poorly under-stood compared with those over mitosis. Here, we show that S.pombe mei2, which is essential for the initiation of premeiotic DNA synthesis, encodes an RNA-binding protein. A temperature-sensitive mei2 mutant performs premeiotic DNA synthesis but does not undergo meiotic divisions, suggesting thet Mei2 is required also for meiosis I.A novel, polyadenylated RNA species (meiRNA) , which suppresses this temper-ature-sensitive defect if overexpressed, specifically binds to Mei2 both in vivo and in vitro. Cells without meiRNA perform premeiotic DNA synthesis but cannot undergo meiosis I.Mutations that apparently block the RNA binding ability of Mei2 inhibit premeiotic DNA syn-thesis. Mei2 is thus likely to couple with another RNA species to promote premeiotic DNA synthesis.
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