| Project/Area Number |
05680632
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
OKADA Masukichi Univ.of Tsukuba, Inst.of Biol.Sci., Prof., 生物科学系, 教授 (60015534)
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| Co-Investigator(Kenkyū-buntansha) |
MARUO Fumiaki Univ.of Tsukuba, Inst.of Biol.Sci., Research Associate, 生物科学系, 助手 (30199921)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Drosophila / Oogenesis / Spermatogenesis / Oogonium / Spermatogonium / Cytoplasmic Bridge / Polyfusome / Stem Cell / 不完全分裂 |
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| Research Abstract |
In many animals gametogenesis takes place in germline cysts. Drosophila oocytes develop within cysts containing 16 oogonial cells that are interconnected by intercellular bridges. A cytoplasmic structure, polyfusome, extends through each intercellular bridge in the cyst. To gain insight into the role of polyfusome in controlling the cyst formation and the cytoplasmic asymmetry within each cyst, we have carried molecular analyzes on a component of Drosophila polyfusome using a monoclonal antibody. New findings obtained in this study are as follows.
1. Polyfusome contained cytoskeletal proteins.
2. The unegual distribution of the fusomal component was found in the early ovarian cysts. This is the earliest molecular asymmetry observed in the cyst.
3. Polyfusome was found in the male germline cells as well as in the female germline cells.
4. Morphological differences between male and female polyfusomes were observed. These differences were under the control of the germline sex determination genes.
5. The fusomal component was also contained in male and female germline stem cells. The cellular polarity in stem cell could be recognized by the asymmetrical distribution of the fusome in the cell.
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