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Isolation and characterizasion of genes required for cytokinesis.

Research Project

Project/Area Number 05680637
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KUBOTA Hiroshi y.  Kyoto University, Lecturer Faculty of Science,, 理学部, 講師 (40115837)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordscytokinesis / cleavage / a maternal effect mutant / Xenopus laevis / 卵割溝 / 小麦胚芽レクチン
Research Abstract

Females of a maternal-effect mutant (af) of Xenopus laevis lay eggs whose cleavage furrows do not contract. A small amount of cytoplasm from wild-type eggs, when injected into af eggs, induces contraction of the cleavage furrow in af eggs. This enabled us to isolate contraction-inducing substances from wild-type eggs. The precipitate of 0-40% saturated ammonium sulfate fraction was separated by DEAE ion-exchange column chromatography. Fractions having contraction-inducing activity were collected and re-separated by the same column. Comparing the pattern of SDS-PAGE of each fraction and contraction-inducing activity, we found that 31 kDa and 33 kDa bands were concentrated in the fractions which share the contraction-inducing activity. Further separation by gel-filtration column chromatography showed that active components form very high molecular weight complexes. We are making preparations for isolating genes which encode these proteins by making monoclonalantibodies or by analysing the amino acid sequences of these proteins.
In order to maintain af genes, siblings of mutant females were inbred. Among the descendants, we found a female which lays eggs whose cleavage furrows were abnormal. About a half of the eggs layd by the female were lethal because of the incomplete cytokinesis at the first and second cleavages. This abnormality may be due to insufficiency or functional impediment of a maternal product which is differnt from af gene product.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Hiroshi Y. Kubota: "Oscillation of intracellular free calcium in cleaving and cleavage-arrested embryos of Xenopus laevis." Developmental Biology. 160. 512-518 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroshi Y. Kubota: "A female sterile mutant,unfertilizable(uf),in Xenopus laevis." Developmental Biology. 158. 502 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 久保田洋: "アフリカツメガエルの細胞質分裂突然変異" 細胞. 26. 190-194 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroshi Y.Kubota: "A female strile mutant, unfertilizable (uf) , in Xenopus laevis." Developmental Biology. 158. 496-502 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroshi Y.Kubota: "Oscillation of intra-cellular free calcium in cleaving and cleavage-arrested embryos of Xenopus laevis." Developmental Biology. 160. 512-518 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroshi Y.Kubota: "A cleavage-deficient mutant of Xenopus laevis. (in Japanese)" The Cell. 26 (5). 22 (190) -26 (194) (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroshi Y.Kubota: "A female sterile mutant,unfertilizable (uf),in Xenopus laevis." Developmental Biology. 158. 496-502 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiroshi Y.Kubota: "Oscillation of intracellular free calcium in cleaving and cleavage-arrested embryos of Xenopus laevis." Developmental Biology. 160. 512-518 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 久保田 洋: "アメリカツメガエルの細胞質分裂突然変異" 細胞. 26. 22-26 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiroshi Y.Kubota: "A female sterile mutant,unfertilizable(uf),in Xenopus laevis." Developmental Biology. 158. 496-502 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Hiroshi Y.Kubota: "Oscillation of intracellular free calcium in cleaving and cleavage-arrested embryos of Xenopus laevis." Developmental Biology. 160. 512-518 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 久保田 洋: "アフリカツメガエルの細胞質分裂突然変異" 細胞. 26(5) (印刷中). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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