• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Role of zebrafish POU-domain gene, ZFPOU2, in early development

Research Project

Project/Area Number 05680648
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionNagoya University (1994-1995)
The Institute of Physical and Chemical Research (1993)

Principal Investigator

TAKEDA Hiroyuki  Nagoya University, School of Science Associate Professor, 理学部, 助教授 (80179647)

Co-Investigator(Kenkyū-buntansha) 天沼 宏  理化学研究所, 安全評価研究室, 主任研究員 (70107382)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
KeywordsPOU transcription factor / Zebrafish / Gastrulation / Mesoderm induction / ES cell / ES 細胞 / oct-3 / POU / 発生学 / 分子生物学 / POUファミリー / Oct-3
Research Abstract

POU-domain proteins are a large family of transcriptional regulatory proteins, many of which are implicated in the control of gene expression during early development. We describe here the cloning and expression of zebrafish pou2, a novel POU-domain gene related to the mouse germ line-specific transcription factor Oct-3. Zebrafich pou2 is maternally expressed and the transcripts are present from the one-cell stage to the gastruls stage. In situ hybridization analyzes revealed that the transcripts were present in all blastomeres until the midblastula stage and that the expression was restricted to the epiblast during gastrulation. We found that alternatively spliced transcripts, t-pou2 RNA,were also expressed in the embryos. In contrast to the Pou2 product, t-Pou2 product lacks DNA-binding activity because of its incomplete POU-domain structure. To examine the roles of the Pou2 and t-Pou2 products, we increased their expression in the embryo by microinjection of synthetic pou2 and t-pou … More 2 RNAs into the fertilized eggs at the one-cell stage. Most embryos that developed from the eggs injected with pou2 RNA did not show any obvious developmental defects. In contrast, overexpression of the t-Pou2 product greatly affected the embryonic development : there was strong developmental retardation or arrest due to the incomplete gastrulation. In the affected embryos, expression of zebrafish T gene was reduced and the hypoblast formation was disturbed. Temporal and spatial expression patterns and the effects of overxpression of these products on development are consistent with the idea that the Pou2 and t-Pou2 proteins are involved in early development of zebrafish embryos. They may be involved in the proliferation of blastomeres in undetermined state at the blastula stage and/or the early cell commitment events at the gastrula stage. Also, our results indicate that different products generated as a result of alternative splicing from the same gene possess distinct functional capacities. Less

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 武田 洋幸: "ゼブラフィッシュ初期発生とPOU転写因子" 実験医学. 11. 1619-1625 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroyuki Takeda: "A novel POU domain gene,zebrafish pou2 : expression and roles of two alternatively spliced twin products in early development" Genes & Development. 8. 45-59 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroyuki Takeda: "Commitment of cell fate in embryo of the zebrafish,Danio rerio" The Fish Biology Journal MEDAKA. 6. 33-45 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 天沼 喜美子: "ゼブラフィッシュにおける外来遺伝子導入系" 蛋白質核酸酵素. 40. 2257-2264 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Terumi Miyagawa: "Specification of posterior midbrain region in zebrafish neuroepithelium" GENES TO CELLS. 1. 369-377 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshiro Mizuno: "Mesoderm induction in zebrafish" Nature. 383. 131-132 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeda, H., Matsuzaki, T., Oki, T.& Amanuma, H.: "A novel POU domain gene, zebrafish pou2 : expression and roles of two alternative spliced twin products in early development." Genes & Development. 8. 45-59 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takeda, H.& Miyagawa, T.: "Commitment of cell fate in embryo of the zebrafish, Danio rerio. (review article)." The Fish Biology Journal MEDAKA. 6. 33-45 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Miyagawa, T.Amanuma, H., Kuroiwa, A.& Takeda, H.: "Specification of posterior midbrain region in zebrafish neuroepithelium." GENES TO CELLS. 1. 369-377 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Mizuno, T., Yamaha, E., Wakahara, M., Kuroiwa, A.& Takeda, H.: "Mesoderm induction in zebrafish." Nature. 383. 131-132 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 天沼,喜美子: "ゼブラフィッシュにおける外来遺伝子導入系" 蛋白質核酸酵素. 40. 2257-2264 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Terumi Miyagawa: "Specification of posterior midbrain region in zebrafish neuroepithelium" GENES TO CELLS. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroyuki TAKEDA: "A novel POU domain gene,gene,Zeboatlsh pou2:expression and roles of two altenatiuely spliced twin products in early develpment." Genes & Developrcent. 8. 45-59 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiroyuki TAKEDA: "Comnitment of cell fate in emboyo of the zevntish Danto rerio." The Fish Biology Journal MEDAKA. 6. 33-45 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ishizuya-Oka,Atsuko: "Cell-specitic and Spacio-temporal expression of intestinal fatty acid-bindioy portein gene duriy amphibian metam-cphosis" Roux´s Archiates Developmental Biology. 204. 150-155 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 武田洋幸: "コールドスプリングハーバーLab.ミーティング「Zebratlsh Development & Genetics」に参加して" 実験医学. 12. 1676-1677 (994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 武田洋幸: "実験動物としてのゼブラフィッシュと遺伝子導入" Molecular Medicine 増刊号「疾患モデルマウス」. 21- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiroyuki Takeda: "A novel POU domain gene,zebrafish pou2:expression and roles of two alternatively spliced twin products in early development" Genes & Development. 8. 45-59 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 武田 洋幸: "ゼブラフィッシュ初期発生とPOU転写因子" 実験医学 増刊. 11. 1619-1625 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 武田 洋幸: "ゼブラフィッシュ-中枢神経系発生の脊椎動物モデルとして" 実験医学. 11. 65-68 (1993)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi