| Project/Area Number |
05680651
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Nerve anatomy/Neuropathology
|
|---|
| Research Institution | CHIBA UNIVERSITY |
|---|
Principal Investigator |
TANAKA Koichi Chiba Univ., School of Med, Assistant, 医学部, 助手 (20125941)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Project Status |
Completed (Fiscal Year 1994)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | Nitric oxide / Nitric oxide synthase / Cardiac ganglia / guinea pig / immunohistochemistry / conductive tissue / 電子顕微鏡 |
|---|
| Research Abstract |
Immunoelectron microscopy was performed to localize immunoreactivity for nitric oxide synthase (NOS) in guinea pig heart. Many round intracardiac ganglia, close to the openings of the pulmonary veins, contained NOS-immunoreactive neurons. A small number of neurons were immunoreactive, but most of the non-immunoreactive neurons were surrounded by immunoreactive axons. Direct axo-somatic or-spinic synapses from NOS-immunoreactive axon varicosities to intracardiac ganglionic neurons were observed. Many small ganglia were found in the interatrial septum near the atrioventricular node. A small number of neurons in these ganglia were also immunoreactive. NOS immunoreactive axons were observed in nerve bundles near or within the node. Very thick immunoreactive axons, 8-10 mum in diameter, were found between the conductive tissue and the ordinary cardiac muscles. Electron-microscopic examination confirmed that these axons were unmyelinated. Nerve bundles in this region contained both myelinated and unmyelinated axons ; however, immunoreactivity was detected exclusively in unmyelinated axons. Fine NOS-immunoreactive nerve fibers with terminal varicosities were often seen in the atrioventricular node. Close contact between NOS-immunoreactive axon varicosity and specialized cardiac muscle cell was observed. These results indicate a possibility that nitric oxide plays a role in the neural control of the conductive tissue in the heart through direct neuromuscular contact.
|
