cDNA cloning of novel seven-transmembrane receptors from rat brain
| Project/Area Number |
05680682
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | KYOTO UNIVERSITY (1994)
Himeji Institute of Technology (1993) |
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Principal Investigator |
HATA Shingo KYOTO UNIV., FAC.AGRI., ASSISUTANT PROFESSOR, 農学部, 助教授 (40238001)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | seven-transmembrane receptor / Gprotein-coupled receptor / PCR / cDNA cloning / brain / G蛋白質共役型受容体 / ラット |
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| Research Abstract |
Using degenerate oligonucleotide primers corresponding to conserved regions of the G-protein coupled receptor (seven-transmembrane receptor) superfamily, we carried out a low-stringency polymerase chain reaction and obtained two novel partial-length clones from a rat brain cDNA library. We used one of these clones for conventional library screening and isolated a longer cDNA clone, designated as RBU-15, from another rat brain library. Although RBU-15 was truncated at its 5' end, Northern blot analysis revealed that the gene was expressed in the brain and spleen. Next, we isolated a full-length cDNA clone, designated as HB-954, from a human fetal brain library, using RBU-15 as a probe. The deduced amino acid sequence of HB-954 contained four putative glycosylation sites in the N-terminal part, seven transmembrane domains, and a large cytosolic domain in the C-terminal part. The protein products of RBU-15 and HB-954 likely belong to a distinctive subfamily, because no receptors in the superfamily were found to be closely related to them.
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Report
(3 results)
Research Products
(12 results)
