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Role of immunoglobulin superfamily adhesion molecule F3/F11 at the late stage of neural development.

Research Project

Project/Area Number 05680689
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurochemistry/Neuropharmacology
Research InstitutionTokyo Metropolitan Institute of Gerontology

Principal Investigator

WATANABE Kazutada  Tokyo Metropol.Inst.of Gerontol., Dept.of Exp.Biol., Head, 実験生物学部門, 室長 (70114717)

Co-Investigator(Kenkyū-buntansha) HOSOYA Hiroko (KASUGA Hi)  Tokyo Metropol.Inst.of Gerontol., Dept.of Exp.Biol., Research assistant, 実験生物学部門, 助手 (00158841)
KOBAYASHI Satoru  Tokyo Metropol.Inst.of Gerontol., Dept.of Exp.Biol., Researcher, 実験生物学部門, 研究員 (20100117)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsF3 / F11 / Contactin / Immunoglobulin superfamily / GPI-anchor / Neural adhesion / In situ hybridization / Brain development / コンタクチン / 免疫グロブリン・スーパーファミリー / GPIアンカー / In sitn ハイブリダイゼーション / 免疫クロブリン・スーパーファミリー / 分子生物学 / 神経発生 / 学習
Research Abstract

We cloned a bovine cDNA encoding the neural adhesion molecule F3 and analyzed its nucleotide sequence. The coding region consisted of 3054 bp encoding 1018 amino acid residues. The Mr calculated from the deduced amino acid sequence was 113383. Bovine F3 had 93,94 and 77% amino acid identities with the mouse, human and chicken homologs, respectively. Parts of bovine F3 protein were overporduced in Escherichia coli. The antibodies raised against the recombinant proteins in rabbits reacted specifically with F3. F3 protein was detected in cerebellum, cerebrum and spinal cord in Western blot analysis. Then we cloned a cDNA encoding rat F3 to elucidate the developmental changes of F3 in the rat brain. During postnatal development of the rat brain, cells expressing F3 mRNA appeared in the cortex, hippocampus, superior and inferior colliculi, anterior olfactory nucleus, olfactory bulb and cerebellum, whereas little was observed at postnatal day 1 (P1). Extraordinarily high expression of F3 mRNA was observed in the cerebral cortical neurons of layr 5 of at P7. The number of cells with high expression of F3 mRNA expanded to the entire region of the cerebral cortex at P14. The whole cerebrum displayd the expression at P90 in which the cortex still showed the highest expression level, although the overall signals were weak in comparison with those at P14. In the hippocampal formation, F3-expressing granule cells of the dentate gyrus were restricted to the outer aspect, then expanded to the inner aspect during development. Finally the granule cells in the entire region of the dentate gyrus transcribed F3 mRNA.We discussed the significance of the expression pattern of F3 mRNA during development.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Shimazaki, K.: "F3/F11 expression in the developping rat brain" Jpn. J. Physiol. supl.44. s264 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimazaki, K.: "BCL-2 oncoprotein change and the tolerance to ischemia induced neuronal death in the gerbil hippocampus" Neurosci. Abstr.20. 685 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hosoya, H.: "Developmental expression of the neural adhesion molecule F3 in the rat brain" Neuroscience Letters. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe, K: "Molecular cloning of the cDNA encoding F3 from the bovine brain" Gene. (印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimazaki, K., Ishida, A., Kasuga, H., Kobayashi, S., Watanabe, K.and Kawai, N.: "F3/F11 expression in the developping rat brain." Jpn J.Physiol.supl.44. s264 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimazaki K., Ishida, A., Watanabe, K.and Sahara, Y.: "BCL-2 oncoprotein change and the tolerance to ischemia induced neuronal death in the gerbil hippocampus." Neurosci.Abstr.20. 685 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hosoya, H., Shimazaki, K., Kobayashi, S., Shirasawa, T., Takenawa, T.and Watanabe, K.: "Developmental expression of the neural adhesion molecule F3 in the rat brain." Neuroscience Letters. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Watanabe, K., Shimazaki, K., Hosoya, H., Kobayashi, S., Fukamauchi, F.and Takenawa, T.: "Molecular cloning of the cDNA encoding F3 from the bovine brain." Gene. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takeuchi, K., Kimura, M., Sato, N., Tsukita, S.and Watanabe, K.: "Perturbation of cell adhesion and axonal growth by antisense oligonucleotides to cell adhesion and its undercoat molecules." First Internation0al Antisense Conference, Kyoto, 1994.12.4-7.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hosoya,H.: "Derelopmental expression of the neural adhesion molecule F3 in the rat brain" Neuroscience Letters. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Watanabe,K.: "Molecular cloning of the cDNA encoding F3 from the bovine brain" Gene. (印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 島崎久仁子: "神経特異的細胞接着因子F3/F11の局在性" 生化学. 65. 848 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 春日博子: "神経細胞接着因子F3/F11のcDNAのクローニングと脳内での発現" 第16回日本分子生物学会年会・講演要旨集. 364 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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