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Studies on functional properties of the inhibitory neurons in the hippocampus of the rat using slice-patch techniques and newly modified pr ocedur es.

Research Project

Project/Area Number 05680714
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 神経・脳内生理学
Research InstitutionTohoku University

Principal Investigator

KAWA Kazuyoshi  Tohoku Univ.School of Medicine, Associate professor, 医学部, 助教授 (70125839)

Co-Investigator(Kenkyū-buntansha) 赤池 紀生  九州大学, 医学部, 教授 (30040182)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsSlice-patch / Hippocampus / Inhibitory neurons / Serotonin / Receptor / Rat / ニューロン / GABA / イオンチャネル / パッチクランプ
Research Abstract

In dentate gyrus of the rat hippocampal slices, principal neurons (granule cells) and local inhibitory neurons (basket cells) identified under Namarsky microscopy. The effects of serotonin (5-HT) on these neurons were investigated using the whole-cell patch clamp technique and rapid perfusion (Y-tube) method. In granule cells, external application of 5-HT (0.2-100 uM) did not evoke any current but in basket cells definite inward currents at a holding potential of -63mV were induced. The I-V relation for 5-HT response showed a reversal potential around 0 mV accompanying a negative slope conductance at potentials below -60mV.
To compare the functional proparties of these 5-HT receptors, newly identified ADP (adenosine-5'-diphosphate) receptors in the megakaryocytes from the mouse bone marrow were studied. When Cs^+ was used as a major internal cation to block K-currents, external application of ADP (10-100 uM) to dissociated megakaryocytes induced large inward currents (amplitude of 100-500 pA) at a holding potential of -42 mV,which showed a rapid decay with a complete inactivation in 1 sec.
The amplitude of the ADP-induced currents became larger when hyperpolarized, while it became smaller by depolarization and reversed its polarity to outward at aroudn 0 mV.The overall I-V relationship showed an inward rectification. The ADP-induced inward currents are also observed in isotonic Ca2+ or Ba2+ or Mg2+ solution, which indicates that the channels activated by ADP are pormeable to these divalent cations. The channels are also permeable to monovalent cations such as Na+, K+ or Cs+.
These indicate that a certain type of purinergic receptor is specifically involved in generating rapid cationic currents in megakaryocytes, the functional properties of which are very similar to those described for the 5-HT receptors in the brain.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Kawa,K.: "Distribution and functional properties of 5-HT_3 receptors in the rat hippocampal dentate gyrus: a patch-clamp study." J.Neurophysiol.71. 1935-1947 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawa,K.: "Inhibitory effect of methylxanthies on glycine-induced Clcurrent in dissociated rat hippocampal neurons." Ann N.Y.Acad.Sci.707. 449-453 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akaike,N.: "Existence of rolipram-sensitive phosphodiesterase in rat megakaryocyte." Br.J.Pharmacol.109. 1020-1023 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawa,K.: "ADP-operated rapid inward currents observed in dissociated mouse megakaryocyte." Neuroscience Research. S19. s50- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 河 和善(共著): "“ブレインサイエンス最前線 '95" 佐藤昌康編" 講談社サイエンティフィク, 138 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawa, K.: "Distribution and functional properties of 5-HT3 receptors in the rat hippocampal dentate gyrus : a patch-clamp study." J.Neurophysiology. 71. 1935-1947 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawa, K.et al.: "Inhibitory effect of methylxanthines on glycine-induced Cl-current in dissociated rat hippocampal neurons." Ann New York Acad.Sci. 707. 449-453 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akaike, N.et al.: "Existence of rolipram-sensitive phosphodiesterase in rat megakaryocyte." Br.J.Pharmacol.109. 1020-1023 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawa, K.: "ADP-operated inward currents observed in dissociated mouse megakaryocytes." Neuroscience Research. S19. s50 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawa, K.: The ion-channels and receptors in the brain : their regulated expression and functions.In : Frotiers in Brain Research '95, Ed.Sato, M.Kohdan-sha Scientific (Tokyo), 1-138 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawa,K.: "Distribution and functional properties of 5-HT_3 receptors in the rat hippocampal dentate gyrus:a patch-clamp study." J.Neuropysiol.71. 1935-1947 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawa,K.: "Inhibitory effect of methylxanthines on glycine-induced Clcurrent in dissociated rat hippocamal neurons." Ann N.Y.Acad.Sci.707. 449-453 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Akaike,N.: "Existence of rolipram-sensitive phosphodiesterase in rat megakaryocyte." Br.J.Pharmacol.109. 1020-1023 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawa,K.: "ADP-operated rapid currents observed in dissociated mouse megakaryocyte." Neuroscience Research. S19. S50 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 河 和善(共著): "“ブレインサイエンス最前線'95"佐藤昌康編" 講談社サイエンティフィク, 138 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Akaike,N.: "Existence of rolipram-sensitive phosphodiesterase in rat megakaryocyte." Br.J.Pharmacology. 109. 1020-1023 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kawa,K.: "Effects of curare on serotonin type 3 receptor (5-HT_3R)-mediated response in rat hippocampal dentate neurones;a slice-patch study." Neuroscience Res.18. S22 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kawa,K.: "Inhibitory effect of methylxanthines on glycine-induced Cl-current in dissociated rat hippocampal neurons." Annals of the New York Acad.Sci.(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kawa,K.: "Distribution and functional properties of 5-HT_3 receptors in the rat hippocampal dentate gyrus:a patch-clamp study." J.Neurophysiology. (in press). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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