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ON THE SIGNAL TRANSDUCTION MECHANISMS AT THE SECONDARY AND THE TERTIARY COCHLEAR NUCLEL

Research Project

Project/Area Number 05680719
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 神経・脳内生理学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

KOYANO Konomi  KYOTO UNIVERSITY.DEPT.OF PHYSIOLOGY.INSTRUCTOR, 医学部, 助手 (50183041)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsCochlear nucleus / N.magunocellularis / Membrane properties / Jonic currents / Sound localization / A-電流 / グルタミン酸 / γ-アミノ酪酸
Research Abstract

Avian magno cellular nucleus (NMC) is one of cochlear nuclei which is known to encode the temporal information of sound. The higher auditory nuclei are supposed to detect interaural time difference to locate a sound origin using the temporal information from NMC.In this project, we investigated the membrane properties and ionic currents of the NMC neuron which enable to carry the temporal information of sound using a whole-cell recording form brain stcm slice preparation of a chick embryo. Under current-clamp condition, NMC neuron showed Na-dependent action potentials with a threshold of about-44mV.The neuron generated only a single action potential with a fixed timing at the initial phase of depolarizing current stimuli. This excitable nature was independent from the intensity and the duration of the current stimuli. This characteristic excitability is seemed to be useful to carry the temporal information of the sound. In voltage-clamp condition, the neurone revealed a TTX-sensitive Na current, a low voltage activated (LVA) Ca current and a high voltage (HVA) Ca current. The LVA Ca current and the HVA Ca current were sensitive to Ni (0.1mM) and nifedipime (20 muM) , respectively. The neuron showed at least a TEA-sensitive K.current and a 4-AP-sensitive K current. In the presence of 4-AP (0.5 mM) , the NMC neuron generated multiple action potentials from a lower threshold, in response to long depolarizing current injections. The onset of spikes showed marked fluctuation at smaller current injections. Ni (0.1 mM) prevented effects of 4-AP on the threshold value and the variability of action potential onsets. In voltage clamp experiment, 4-AP uncovered the LVA Ca current at more negative potential than the normal action potential threshold of-44mV.We have conchuded that a 4-AP-sensitive K current overcomes the LVA Ca current to set a higher threshold level to generate a single action potential at the initial phase of synaptic inputs.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Koyano, K.: "The role of arachidonic and metabolism in somafostatin-and substomce P-induced modulation of the inward rectifying potassium conductonce in rat brain neurones." Neuroscience Research. 20. 345-354 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Velimirovic. B.M.: "Opposing wechanisms of requlation of an inward rectifier Kchannel in brain neurons" Pioc. Natl. Acad. Sci USA. in press. (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koyano, K.et al: "The role of arachidonic acid metabolism in somatostatin-and substance P-induced modulation of inwardly rectifying potassium conductance in rat brain neurons" Neuroscience Research. 20. 345-345 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Velimirovic, B.M.et al: "Opposing echanism of regulation of an inward rectifier K channel in brain neurons" Proc.Natl.Acad.Sci.USA. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koyano,K.: "The role of arachidonic acid metabolism in somatostatin-and substance P-induced modulation of the inward rectifying potassiom conductance in rat brain neurons." Neuroscience Research. 20. 345-354 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Velimirovic,O.M.: "Opposing mechanisms of reguration of an inward rectifier K channel in brain neurons" Proc.Natl.Acad.Sci,USA. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Koyano,K.: "Membrane properties of the neuron of the chick auditory nucleus:Nucleus magunocellularis." Neuroscience Reserch. suppl:18. s190 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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