| Project/Area Number |
05680745
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Central Institute for Experimental Animals (CIEA) |
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Principal Investigator |
ITOH Toshio CIEA, Laboratory Animal Medicine, Chief, 動物医学研究室, 室長 (20106644)
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| Co-Investigator(Kenkyū-buntansha) |
OHASHI Hiroaki CIEA, Laboratory Animal Medicine, Researchier, 動物医学研究室, 研究員 (30250012)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | CAR bacillus / Improvement of culture method / Pathogenesis / カ-バチルス / 実験動物 / 培養方法 / 感染病 / マウス / 細菌 |
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| Research Abstract |
Cilia-associated respiratory (CAR) bacillus is an unique respiratory pathogen for laboratory animals such as mice, rats and rabbits, which is difficult to culture. Because of the difficulty of propagation of this organism, there are few studies concerning its pathogenesis. The purposes of this study were improvement of the culture methods and elucidation of pathogenic factors.
For improvement of the culture methods, embryonated chicken eggs and in vitro cultures were studied. Embryonated chicken egg-associated CAR bacillus was not established by passaging. Changes in the components of the culture medium and the use of a culture bottle courted with chemical substances did not affect growth.
Concerning the pathogenicity of this organism, repeated administration of a large number of killed organisms did not produce any lung lesions in mice. Because we failed to improve cultures of this organism, and could not obtain sufficient amounts of CAR bacillus, studies on pathogenicity were inadequate.
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