STUDY OF BIODEGRADABLE MATERIALS FOR ANTI-ADHESIVE MEMBRANE IN VIVO
| Project/Area Number |
05680762
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | NARA MEDICAL UNIVERSITY |
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Principal Investigator |
YAJIMA Hiroshi ASSISTANT PROFESSOR NARA MEDICAL UNIVERSITY, 医学部, 講師 (20221640)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Yurito LECTURER NARA MEDICAL UNIVERSITY, 医学部, 助手 (70203453)
TAMAI Susumu PROFESSOR NARA MEDICAL UNIVERSITY, 医学部, 教授 (10075088)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | TENDON / ANTI-ADHESIVE MATERIAL / ADHESION / GELATIN / ENDOTOXIN |
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| Research Abstract |
In the case of flexor tendon injury alone, adhesion can be prevented by early controlled mobilization. But immobilization for 4 weeks is required for a badly injured tendon with the tendon sheath defects. Crosslinked gelatin was developed to apply such cases as anti-adhesive membrane. In this study we observed the reactions of the membrane for 8 weeks in rats and the effects on reduction of adhesion for 4 weeks after tendon surgery in chickens.
Materials and methods : Gelatin was crosslinked under the condition of vacuum, low pressure and 150ﾟC for 24 hours. It was made using two kinds of distillled water of different concentrations of endotoxin (endotoxin (+), (-)). The gelatin sheets were implanted in the back of rats subcutaneously. They were sacrificed at 2,4,7,10 and 14 days after implantation and histological study was performed. At 4,7 and 10 days postoperatively, infiltration of leukocyte was seen in around endotoxin (+) specimen. But its reaction was not seen around endotoxin (-) specimen until 14 days.
Then the chickens aged 3 to 4 months were used. On the left third toe, the tendon sheath was removed, the flexor tendon was cut and the bony floor was injured. The tendon was sutured and wrapped around with the gelatin membrane. On the right toe, the tendon was sutured alone. The chickens were sacrificed at 2 and 4 weeks postoperatively. The toes were studied histologically and dynamic study was also performed. In the histological study, adhesion was eliminated effectively by using the membrane. In the dynamic study, there was no significant difference between the two groups at 2 and 4 weeks.
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Report
(4 results)
Research Products
(9 results)
