| Project/Area Number |
05680764
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | TOKAI UNIVERSITY |
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Principal Investigator |
YODA Kentaro TOKAI UNIV., BIOMEDICAL ENGINEERING,PROFESSOR, 開発工学部, 教授 (80240365)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Kazuo TOKAI UNIV., BIOMEDICAL ENGINEERING,ASSOCIATE PROFESSOR, 開発工学部, 助教授 (10112781)
SAKAKIBARA Manabu TOKAI UNIV., BIOLOGICAL SCIENCE AND TECHNOLOGY, PROFESSOR, 開発工学部, 教授 (10135379)
HORIKAWA Muneyuki TOKAI UNIV., BIOMEDICAL ENGINEERING,PROFESSOR, 開発工学部, 教授 (40229232)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1993: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | DOSAL ROOT GANGLION / CULTURED NEURON / LOW POWER LASER / PATCH-CLAMP METHOD / CALCIUM SIGNALING / PAIN ALLEVIATION / BRADYKININ / ACTION POTENTIAL / 神経細胞培養 / 神経成長因子 / ラミニン / 微細電極基板 / 痛み刺激 |
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| Research Abstract |
Dosal root ganglia dissected from adult mice were subfected to successive digestion first by collagenase and then by tripsin. Dissociated neurons were purified from satelite cells and digestion fragments by percoll density gradient centrifugal process. Purified neurons were seeded on poly-L-lysine and laminin coated polystylene dished and cultured in a serum-containning or serum-free medium. Nerve growth factor enhanced the growth of neulites. Patch-clamp whole-cell method and fluorescence image analysis technique were utilized to determine the action potencial and calcium ion oscillation signals of the cultured neuron evoked by stimulation of bradykinin. Both frequency of action potential and concentration of calcium ion in cultured neuron were increased with stimulation of bradykinin. Ga-Al-As low power laser irradiation to neulites decreased the frequency of action potential.
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