| Project/Area Number |
05804047
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理
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| Research Institution | Tokyo University of Pharmacy and Life Science (1994-1995)
The University of Tokyo (1993) |
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Principal Investigator |
TSUZUKI Mikio Tokyo University of Pharmacy and Life Scinece, School of Life Science, Assistant Professor, 生命科学部, 教授 (70155430)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | pyrenoid / carbon dioxide / microalgae / Chlamydomonas / Rubisco / starch |
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| Research Abstract |
The pyrenoid, which is located in chloroplast of algae, increases under low CO_2 conditions by about 3 times in Chlamydomonas. Sucrose density gradient centrifugation of the pyrenoid fixed with HgC1_2 showed relatively heavier pyrenoid in the cells adapted to low CO_2 condition. Average protein content of the pyrenoid showed the same result. During the adaptation to low CO_2 concentration where the pyrenoid developed, rbcS1, one of two genes of small subunit of Rubisco, was transcribed less than that in high CO_2 condition, while rbcS2, the other Rubisco gene, was at almost the same level. The development of the pyrenoid was still observed in the presence of chloramphenicol.
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