| Project/Area Number |
05806001
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
IKEHASHI Hiroshi Kyoto Univ.Fac.Agriculture, Professor, 農学部, 教授 (50193222)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | self-imcompatibility / Japanese pear / Apple / RNase / Glyco-protein / Breeding |
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| Research Abstract |
Using several varieties of Japanese pear (Pyrus serotina Rehd.) , each of which has known genotype in regards to self-incompatibility, proteins extracted from the stylars were analyzed by two dimensional gel elecctro-phoresis. As the results, self-incompatibility-related RNases which cosegregate with S-genotypes were identified. The RNases were dificient in a self-compatible mutant of Japanese pear. N-terminal aminoacid sequences of the RNases showed clear homology with S-RNases of Petunia.
Applying the above results, self-incompatibility-related glycoproteins in styles of apple (Malus x domestica) were identified. The self-incompatibility genotypes for more than ten varieties were determined.
Self-incompatibility-related RNases in Pyrus communis were studied. Loss of one of S-RNases was confirmed in self-compatible cultivars.
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