| Project/Area Number |
05806007
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | Institute of physical & Chemical Research (RIKEN) |
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Principal Investigator |
MITSUI Takashi RIKEN,Chief Scientist, 昆虫生態制御研究室, 主任研究員 (00087602)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Shogo RIKEN,Senior Research Scientist, 昆虫生態制御研究室, 先任研究員 (60134516)
小澤 理香 理化学研究所, 昆虫生態制御研究室, 奨励研究生
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Insect neuropeptide / Receptor / Pheromone biosynthesis / Signal transduction Silkworm / シグナル伝達 / 神経ペプチドホルモン / アワヨトウ / アワヨトウ体色黒化ホルモン |
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| Research Abstract |
To analyze the receptors of insect neurohormones having FXPRLamide at the C-terminus, we have prepared specific ligands for the receptors. Among the ligands we prepared, tritiated or iodinated probes with radio activity bound to the receptors of melanization and reddish coloration hormone (MRCH) and pheromone biosynthesis activating neuropeptide (PBAN). However, non-specific bindings to these probes were also detected. To eliminate the non-specific bindings, next we tried to design and prepared a non-radio labeled ligand having 3 functional cites (avidin binding cite, covalent bonding cite and receptor binding cite) in the peptide molecule, TKYFSPRLamide. By using this probe, we have established to detect PBAN receptor prepared from the pheromone gland of the silkworm moths.
We have also elucidated the mechanism of intracellular signal transduction triggered by the binding of the neurohormone and its receptor. Our results suggested that external signal of PBAN is transduced by the influx of extracellular calcium ion, calmodulin, and phosphoprotein phosphatse which activates the final step of bombykol biosynthesis catalyzed by acyl CoA reductase.
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