| Project/Area Number |
05807007
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Shimane Medical University |
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Principal Investigator |
SHIKIMI Tadahiro Shimane Med.Univ., School of Med., Associate Professor, 医学部, 助教授 (00065913)
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| Co-Investigator(Kenkyū-buntansha) |
GONDA Tatsuo Shimane Med.Univ., School of Med., Associate Professor, 医学部, 助教授 (40116398)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Ulinastatin-like substance / Mouse brain / Fear-stress / Cerebrospinal fluid / Dementia / Glutamate agonists / Brain ischemia / ウリナスタチン抗体反応陽性物質 / α1-ミクログロブリン抗体反応陽性物質 / パーキンソン氏病 / トリプシン阻害物質 / グルタミン酸受容体 |
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| Research Abstract |
In the present study, a human urinary trypsin inhibitor, ulinastatin (UT) -like immunoreactive substance with trypsin inhibitory activity was demonstrated in certain brain regions in mouse, especially the cerebral cortex and hippocampus. Mechanical lesioning of the cerebral cortex or hippocampus with a needle induced a rapid and intense appearance of a UT-like immunoreactive substance in the neuronal cells of injured sites. Conditioned fear-stress induction incited a reversible increase in the level of UT-like immunoreactive substance in the hippocampus. Glutamate agonists enhanced the levels of this substance in the hippocampus and cerebral cortex. The NMDA induced increase in content of this UT-like substance was antagonized by NMDA antagonists. Twenty-four hr after ischemic injury, a decrease in the level of the substance was observed in the hippocampus, and a recovery tendency was observed at 72 hr post-injury. These results suggest that the UT-like substance in the brain is closely related with the neuronal activity.
The UT-like immunoreactive substance in the cerebrospinal fluid (CSF) was also examined. The level of the substance was lower in patients with dementia than dementia-free subjects, and the level was negatively correlated with the severity of dementia. These results suggest that the UT-like immunoreactive substance in CSF is closely related with dementia.
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