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THE ROLE OF TRANSCRIPTIONAL REGULATION FACTORS ON ONTOGENY,DIFFERENTIATION AND ONCOGENESIS ; ITS APPLICATION FOR HISTOPATHOLOGIC DIAGNOSIS

Research Project

Project/Area Number 05807014
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionKOBE UNIVERSITY

Principal Investigator

HAYASHI Yoshitake  KOBE UNIVERSITY.MEDICINE ASSOCIATE PROFESSOR, 医学部, 助教授 (50189669)

Co-Investigator(Kenkyū-buntansha) OBAYASHI Chiho  KOBE UNIVERSITY.MEDICINE LECTURER, 医学部・附属病院, 講師 (90223940)
HANIOKA Keisuke  KOBE UNIVERSITY.MEDICINE ASSOCIATEPROFESSOR, 医学部・附属病院, 助教授 (70127463)
ITOH Hiroshi  KOBE UNIVERSITY.MEDICINE PROFESSOR, 医学部, 教授 (40168373)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsHNF-1 / vHNF-1 / Competitive RT-PCR / Transcriptional regulation factor / Hepatocellular carcinoma / Hepatoblastoma / Histopathology / Human Pathology / Competetive RT-PCR / 病理 / 転写制御
Research Abstract

The hepatic transcription of human albumin and alpha-fetoprotein (AFP) genes is controlled by a complex set of regulatory elements, and that an AT-rich core sequence (AT motif) is essential as a cis-acting element for the hepatic transcription. Homologous proteins hepatocyte nuclear factor-1 (HNF-1) , variant HNF-1 (vHNF-1) , and AT motif-binding factor 1 (ATBF1) bind to the AT motif. To clarify the role of these three different trans-acting factors, we examined the mRNA and the expression ratio of the HNF-1/vHNF-1 mRNA in various liver tissues. Our competitive reverse transcriptional polymerase chain reaction (RT-PCR) method demonstrated that this ratio is linked to their histological differentiation, and that it is higher in well-differentiated hepatocellular carcinoma (HCC) than in poorly-differentiated and undifferentiated types ; non-neoplastic liver tissues, however, have a low expression ratio of HNF-1/vHNF-1 mRNA.This study indicates that the importance of the HNF-1/vHNF-1 mRNA ratio in determining differentiation of hepatocytic neoplasms and suggests that measuring the ratio would be a useful diagnostic method. Recently, medical electronics have been developed and we must diagnose liver disease by a tiny tissues obtained from echoguided needle biopsy, however a small sized well-differentiated hepatocellular carcinoma is difficult to diagnose differentially from non-neoplastic liver tissues only by histological features. Our competitive RT-PCR method mesuring the ratio is steady and easy to obtain reliable data, using frozen tiny liver tissues, and this, in addition to histological features, must be contributing to the differential diagnosis of well-differentiated HCC from non-neoplastic lesions. We believe that the present work is highly valuable for a medical practice as well as medical science.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report

Research Products

(23 results)

All Other

All Publications (23 results)

  • [Publications] Masahiko Sugano: "Quantitation of Hepatitis C Viral RNA in Liver Tissucs and Sera by Competitive PCR: Relationship with Response to Interferon Alpha Treatment" J Clin Pathol,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshitake Hayashi: "Clinicopathologic and molecular pathologic approaches to Lowe's syndorome." Pediatr Pathol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshitake Hayashi: "Thymoma, its tumorigenesis and progression in relationship with expression of EGF, EGF-Receptor, p53, v-erb and ras p21." Virchows Archiv. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshitake Hayashi: "Expression of EGF, EGF-receptor,p53, v-erbBand ras p21 in colorectal neoplasms by immunostaining paraffin-embedded tissues." Pathol Int. 44. 124-130 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Junko Goji: "Expression of c-ErbB2 in Human Neuroblastoma Tissues, Adrenal Medulla Adjacent to Tumor, and Developing Mouse Neural Crest Cells" Am J Pathol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hiroshi Itoh: "Intrauterine infection: Pathological study on chorioamnionitis and villitis of unknown etiology" Endocrine Journal. 41. S135-S140 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sugano M,Hayashi Y,Yoon S,Kinoshita M,Ninomiya T,Ohta K,Itoh H,Kasuga M: "Quantitative of Hepatitis C viral RNA in Liver Tissues and Sera by Competitive PCR : Relationship with Response to Interferon Alpha Treatment" J Clin Pathol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohtsu M,Hayashi Y,Amatsu M,Itoh H: "Immunological study of p53, EGF,EGF-Receptor, v-erb B and ras p21 in squamous cell carcinoma of hypopharynx" Kobe J Med Sci. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayashi Y,Hanioka K,Kanomata N,Imai Y,Itoh H: "Clinicopathologic and molecular pathologic approaches to Lowe's syndrome" Pediatr Pathol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayashi Y,Ishii N,Obayashi C,Jinnai K,Hanioka K,Imai Y,Itoh H: "Thymoma, its tumorigenesis and progression in relationship with expression of EGF,EGF-Receptor, p53, v-erb B and ras p21" Virchows Archiv. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Goji j, Nakamura H,Ito H,Mabuchi O,Hashimoto K,Sano K: "Expression of c-Erb B2 in human neuroblastoma tissues, adrenal medulla adjacent to tumor, and developing mouse neural crest cells" Am J Pathol. (in pree).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Murayama T,Matsui T,Hayashi Y,Taniguchi M,Ito M,Nakazuka T,Imoto S,Isobe T,Itoh H,Chihara K: "Plasma cell leukemia with myelofibrosis" Ann Hematol. 69. 151-152 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Itoh H,Hanioka K,Obayashi C,Kanomata N,Itoh K,Kuroda N,Hayashi Y: "Intrauterine infection : Pathological study on chorioamnionitis and villitis of unknown etiology" Endocrine J. 41. S135-S140 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hayashi Y,Widjono YW,Ohta K,Hanioka K,Obayashi C,Itoh K,Itoh H: "Expression of EGF,EGF-receptor, p53, v-erb B and ras p21 in colorectal neoplasms by immunostaining paraffin-embedded tissues" Pathol Int. 44. 124-130 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Masahiko Sugano: "Quantitation of Hepatitis C Viral RNA in Liver Tissues and Sera by Competitive PCR : Relationship with Response to Interferon Alpha Treatment." J Clin Pathol. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshitake Hayashi: "Clinicopathologic and molecular pathologic approaches to Lowe's syndrome." Pediatr Pathol. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshitake Hayashi: "Thymoma,its tumorigenesis and progression in relationship with expression of EGF,EGF-Receptor,p53,v-erb and ras p21." Virchows Archiv. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshitake Hayashi: "Expression of EGF,EGF-receptor,p53,v-erb B and ras p21 in colorectal neoplasms by immunostaining paraffin-embedded tissues." Pathol Int. 44. 124-130 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Junko Goji: "Expression of c-ErbB2 in Human Neuroblastoma Tissues,Adrenal Medulla Adjacent to Tumor,and Developing Mouse Neural Crest Cells" Am J Pathol. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Hiroshi Itoh: "Intrauterine infection : Pathological study on chorioamnionitis and villitis of unknown etiology" Endocrine Journal. 41. S135-S140 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 二宮俊明: "ヒト肝細胞癌,胎児肝組織における肝特異転写制御因子核蛋白(trans-acting nuclear factor)の発現の検索" 日本病理学会会誌. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] 太田恭介: "AFP産生胃癌における肝特異転写制御因子の解析" 日本病理学会会誌. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] Hayashi Y et al.: "Expression of EGF,EGF-receptor,p53,v-erb B and ras p21 in colorectal neoplasms by immunostaining paraffin-embedded tissues" Pathology International. 44. 124-130 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-03-31   Modified: 2016-04-21  

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