c-DNA cloning of the extended lifespan gene for the nondividing human cells
| Project/Area Number |
05807017
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Okayama University |
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Principal Investigator |
KANO Yoshio Deparment of Cell Biology, Okayama University Medical School Researcch Assosiate, 医学部, 助手 (70116200)
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| Co-Investigator(Kenkyū-buntansha) |
MIHARA Koichiro Department of Cell Biology, Okayama University Medical School Researcch Assosiat, 医学部, 助手 (50229788)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | expression library / cDNA cloning / extended lifespan gene / human cell / neuron / senescence / celluar aging / nondividing young cells / ヒト細胞 / 発見ベクター / 細胞の寿命 |
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| Research Abstract |
Senescence is an important aging process both during development and for homeostasis in most multicellular organisms. In the human body, some cells such as neurons and skeletal muscle cells, do not divide at all ; others, such as liver cells, normally divide only once every year or two ; while certain epithelial cells in the gut divide more than twice aday. We have built up the working hypothesis that the excellent maintenance of nondivideing cells are more important for longer lifespan. To identify the genes for the extended lifespan of the cells, we developed a diffrential screening method using cDNA expression library. This allowed us to isolate from a nondividing young cells derived cDNA library one clone (els-1) that exhibited excellent extended lifespan when in troduced and expressed in neuronal cells.
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Report
(3 results)
Research Products
(15 results)
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[Publications] Miyazaki, M., Mihara, K., Bai, L., Kano, Y., Tsuboi, S., Endo, A., Seshimo, K., Yoshida, T.and Namba, M.: "Immortalization of epithelial-like cells from human liver tissue with SV40 T-antigen gene." Exp.Cell Res.206. 27-35 (1993)
Description
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[Publications] Miyazaki, M., Gohda, E., Mihara, K., Tsuboi, S., Kaji, K., Yamamoto, I.and Namba, M.: "Increase in production of hepatocyte growth factor by human embryonic lung fibroblasts in the process of aging in culture." Exp.Cell Res.212. 22-29 (1994)
Description
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Related Report
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