| Project/Area Number |
05807021
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | University of Shizuoka |
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Principal Investigator |
YANAGIHA Yasutake University of Shizuoka, School of Pharmaceutical Sciences, Department of Microbiology, Professor, 薬学部, 教授 (30046255)
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| Co-Investigator(Kenkyū-buntansha) |
MASUZAWA Toshiyuki University of Shizuoka, School of Pharmaceutical Sciences, Department of Microbi, 薬学部, 助手 (10181645)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Lyme disease / Lyme borreliosis / Borrelia burgdorferi / Borrelia japonica / PCR / Gene diagnosis / Ixodes ovatus / Ixodes persulcatus / B.japonica / B.garinii / B.afzelii / Lyme clisease / Ixodes ovats / RFLP |
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| Research Abstract |
Genetic characterization of Borrelia isolates from the tick, Ixodes ovatus, I.persulcatus and the rodent, Apodemus speciosus in Japan were compared to members of the three genospecies of Borrelia burgdorferi sensu lato ; B.burgdorferi sensu stricto, B.garinii and B.afzelii.DNA relatedness of the I.ovatus isolates with strain belonging to the three genospecies was 50 to 64%.These results suggested that the strains isolated from I.ovatus in Japan differ from the three genospecies and should be classified as a new genospecies of B.burgdorferi sensu lato.We propose that strains isolated from I.ovatus should be classifed as B.jeponica sp.nov.We developed PCR-RFLP method based on the RFLP of amplified products from OspA and OspB gene for determination of genospecies.The method is simple and faster than conventional DNA homology method.
Borrelia subcultured for one year in vitro lost or showed reduced expression of OspC in comparison with the low-passage strains.The low-passage strains efficiently infected outbred mice.In contrast, the incidence of infection with the high-passage strains was low.Isolates from the bladders of mice inoculated with the high-passage strains expressed large amounts of OspC in comparison with those originally inoculated.These results indicated that OspC expression is related to the infectivity of Lyme disease borrelia.
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