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Molecular biological analysis of cytoskeletal associated protein in bile stasis.

Research Project

Project/Area Number 05807049
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionSapporo Medical University, School of Medicine

Principal Investigator

TANDA Mariko  Sapporo Medical University, School of Medicine 1st Deparment od Internal Medicine Instructor, 医学部, 助手 (40240931)

Co-Investigator(Kenkyū-buntansha) ITOH Fumio  Sapporo Medical University, School of Medicine 1st Deparment of Internal Medicin, 医学部, 助手 (90223180)
谷内 昭  札幌医科大学, 学長 (50045324)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsBile stasis / Cytoskeletal protein / Protein tyrosine phosphatase / PTPH1 gene / PTPH1 / c-myc / RT-PCR法 / 染色体9番長腕31領域
Research Abstract

We priviously cloned a new protein tyrosine phosphatase from the cDNA library of gastrointestinal tract which is identical to PTPH1 gene isolated by Tonks et al.(Tumor Biol., 13 : 180-186).
PTPH1 gene has a unique domain homologous to cytoskeletal associated protein as well as phosphatase domain. In this study, we have analyzed PTPH1 protein and the tyrosyl phosporylation level on the bile canaliculus fraction of hepatocytes where a large number of cytoskeletal proteins are involved. Expression of PTPH1 mRNA is investigated in various kinds of cell lines and revealed that PTPH1 mRNA is expressed ubiquitously. Expression of PTPH1 is increased by one of the differentiating agent, sodium butyrate, suggesting that PTPH1 play arole in cell growth supression.(Int.J.cancer, 55 : 947-951).PTPH1 mRNA is expressed in hepatocellular carcinoma, however no genetic abberation was not found (J.Gastroenterol., 29 : 727-732). Western blot analysis with the polyclonal anti-serum against PTPH1 protein revealed that the band is observed in bile canaliculus fraction of hepatocytes. However, tyrosyl pohporylation level is not changed in the reconstituted bile canaliculus of rat primary culture hepatocytes by traetment with chrolpromazine, the agent causes the bile stasis.
The effect of other agents on tyrosyl pohsporylation level was now undergoing in our laboratory.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Itoh,F.,Ikuta,S.,Hinoda,Y.,et al.: "Expression and chromosomal assignment of PTPH1 gene encoding a cytosolic protein tyrosine phosphatase homologous to cytoskeletal-associated proteins." Int.J.Cancer. 55. 947-951 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sakamoto,H.,Itoh,F.,Tanda,M.,et al.: "Study of phosphtyrosine level in peri-bile canaliculi." Inter.Hepatol.Comm.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sakamoto, H.et al.: "Study of phosphotyrosine level in peri-bile canaliculi." Int.Hepatology Comm. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Itoh F,et al.: "Expression and chromosomal assignment of PTPH1 gene encoding a cytosolic protein tyrosine phosphatase homologous to cytoskeletal associated proteins." Int.J.Cancer. 55. 947-951 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Arimura,Y.,Tanda,M.,et al.: "cDNA cloning of new protein tyrosine phosphatases in human colon." Tumor Biol.13. 180-186 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] Itoh,F.,Tanda,M.,et al.: "Expression and chromosomal assignment of PTPH1 gene encoding a cytosolic protein tyrosine phosphatase homologous to cytoskeletal associated proteins" Int.J.Cancer. 55. 947-951 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ikuta,S.,Tanda,M.,et al.: "Expression of cytoskeletal-associated protein tyrosine phosphatase PTPH1 mRNA in human hepatocellular carcinomas." J.Gastroenterol.29. 727-732 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tanda,M.et al.: "Expression of cytoskeletal associated protein tyrosine phosphatase PTPH1 mRNA in human hepatocellular carcinomas." J.Gastroenterology.(発表予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Itoh,F.et al.: "Expression and chromosomal localization of PTPH1 gene encoding a cytosolic protein tyrosine phosphatase homologous to cytoskeletal-associated protein." Int.J.Cancer. 55. 947-951 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Itoh,F.et al.: "Decreased expression of DCC mRNA in human colorectal cancers." Int.J.Cancer. 53. 260-263 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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