| Project/Area Number |
05807115
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
YAMAOKA Hiroyuki Yokohama City University , School of Medicine, Instructor, 医学部, 講師 (90230317)
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| Co-Investigator(Kenkyū-buntansha) |
WAKASUGI Junichi Yokohama City University, School of Medicine, Assistant, 医学部, 助手 (00210857)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | TNF / Gradual Release / Fibrin Gel / Lipiodol / Tumor Necrosis factor-alpha |
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| Research Abstract |
To obtain the agents which have gradual release of TNF,TNF was combined with fibrin gel (TNF・FG) or lipiodol (INF・LIP). We investigated these agents have the function of gradual release of TNF and the antitumor effect against metastatic liver tumor. It was confirmed TNF・FG has the gradual release of TNF in vitro using the method of Enzyme-Linked Immuno-Sorbant Assay, and also in vivo in the murine colon 26 subcutaneous tumors by immunohistochemical staining. Further more, it was confirmed TNF・LIP has gradual release of TNF by using the model of murine metastatic liver tumors. The antitumor effect of these agents were significantly stronger than that of only TNF against both murine colon 26 subcutaneous tumors and metastatic liver tumors.
The murine body-weight loss which indicated the side-effect of these agents were also assesed. Although the murine weights loss using TNF・FG were less than using only TNF,TNF・LIP induced loss of weights significantly higher compared with only TNF.
These data suggested that these agents were effective if we used them carefully about those side-effects.
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