Effects of intracerebral transplantation of genetically engineered cells to produce opiates in animal model of chronic pain
| Project/Area Number |
05807127
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Oita Medical University |
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Principal Investigator |
ISONO Mitsuo Oita Medical University, Dept of Neurosurgery, Assistant Professor, 脳神経外科学, 講師 (60151437)
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| Co-Investigator(Kenkyū-buntansha) |
KOHNO Kimitoshi Kyusyu university, School of Medicine, Dept of Biochemistry, Associate Professor, 医学部・生化学, 助教授 (00153479)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | neural transplantation / intractable pain / gene engineering |
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| Research Abstract |
Animal model of chronic pain : Using male adult Wistar rats, the animal model of chronic pain was made by loose ligation of a sciatic nerve.The effects of surgery was determined by hot plate test.We developed the new method to achieve sequential sampling of rat cerebrospinal fluid by inserting special silicone tube into cisterna magna.In the same animal, for consecutive twelve days, the concentration of monoamines in the cerebrospinal fluid could be measured.
Extrinsic control of transfected gene in vivo : We have established a stable transfectant, Nf-1, from normal rat kidney fibroblast transfected with a human metallothionein llA (hMT-llA) promoter/human genomic c-fos fusion gene to produce c-Fos protein.Activation of hMT-llA promoter by heavy metals results in the elevation of fused c-fos gene expression in NF-1 cells.We transplanted these cells in to rat brains and investigated whether activation of hMT-llA might be possible in vivo.We found that Nf-1 cells implanted into rat brains survived and grew well with extrinsic administration of Zinc compared those without Zlnc.Also elevated expression of c-fos mRNA was confirmed in the former group.This fact suggests that hMT-llA could be activated by extrinsic administration of Zinc even in vivo.Thereafter, we also investigated and confirmed the same effects by steroid hormones instead of Zinc.
Establishment of the endorphin secreting cells : This process is now still under going.
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Report
(3 results)
Research Products
(4 results)
