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Development of immunotherapy for brain tumors targeting carbohydrate antigens of glycolipids

Research Project

Project/Area Number 05807129
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionSapporo Medical University School of Midicine, Department of Neurosurgery

Principal Investigator

IBAYASHI Yukihiro  Department of Neurosurgery, Sapporo Medical University, Assistant Professor, 医学部, 講師 (50193636)

Co-Investigator(Kenkyū-buntansha) OHTAKI Masafumi  Department of Neurosurgery, Sapporo Medical University, Assistant Professor, 医学部, 講師 (90168964)
森本 繁文  札幌医科大学, 医学部, 助手 (80174449)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
Keywordsglioma / immunotherapy / GM3 / GM2 / TLC / cell cycle / brain tumor / LAK cells / ganglioside
Research Abstract

The main objective of this project is to develop a novel immunotherapy for gliomas, in which glycolipid antigens are analyzed and quantified systematically and a set of appropriate antibodies would be selected according to the antigenic profile of an individual glioma. We first analyzed glycosphingolipid composition of 11 human glioma cell line, one melanoma cell line, and one fibroblast cell line. The glycosphingolipid composition of glioma cell line are different from that of normal nerve cell. We also found that a human glioma cell line U118MG could modulate glycosphingolipid expression via certain cytodines. We next analyzed the glycolipid antigens of 10 human gliomas, which were revealed to consist mainly of GM3 and GM2 . The reactivity of monoclonal antibodies against those gangliosides was examined next either as chemically isolated antigens or cell surface ones. The intensity of antibody binding was dose-dependent as the gangliosides were prepared on a thin-layr chromatographic plate, On the contrary, antibody reaction was more complex on the cell surface ; GM3 showed positive and negative threshould of antibody binding, and GM2 gave stronger reaction when GM3 co-existed nearly equally on the cell surface. We next analyzed cellcycle related glycolipids expression on a certain glioma. It was revealed that the total glycolipids were expressed nearly twice during mitosis, and nLc4Cer was significantly enhanced during that phase. The results may suggest better antibody react ions against the mitotic cells. In another experiment, we have found that the cell dendity greatly affects the level of antibody binding against GM3. There should be a variety of factors that influence the antibody reactions against glycolipid antigens. We are now focusing on the glycolipid analysis of surgical specimens of glioma tissue as well as on the factors participating in the antibody reactivity against those antigens.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Y.Ibayashi: "Effect of local administration of lymphokine-activated killer cells and interleukin-2 on malignant brain tumor patients" Neurologia medico-chirurgica. 33. 448-457 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 崔 炳 旭: "ヒトグリオーマ細胞T98Gの細胞周期にかかわる糖脂質組成の解析" 神経免疫研究. 6. 200-204 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] B.O.Choi: "Biochemical analysis of cytokine effect on the glycosphingolipid expression of human glioma cell lines" Tumor Res.28. 9-18 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yamaki: "The requirement of host immune response to tumor cells to achieve tumor eradication by intratumoral LAK cell infusion immunotherapy" Oncol.(Life Sci.Adv.). 13. 59-68 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 前田義裕: "ヒトグリオーマ細胞株の糖脂質分析と抗GM3およびGM2抗体の反応性の解析" 神経免疫研究. 7. 89-93 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] B.O.Choi: "Interleukin 4 enhances ganglioside GD3 expression on the human fibroblast cell line WI-38" J.Biochem.117. 315-320 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamaki T.: "Analysis of modulation effect of cytokine on the glycolipid expression of human gliona cell line U118MG." Neuroimmunological Res.5. 139-143 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Suetake K.: "Glycosphingolipid expression on the human glioma cell line U118MG and glioblastoma multiforme." Neuroimmunological Rec. 5. 144-148 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ibayashi Y.: "Effect of local administration of lymphokine activated killer cells and interleukin-2 on malignant brain tumor patients." Neurologia medico-chirurgica. 33. 448-457 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Choi B.O.: "Analysis of glicolipid composition of human glioma cell line T98G related with cell cycle." Neuroimmunological Rec.6. 200-204 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamaki T.: "Analysis of modulatory effect of cytokines on the glycoliopid expression of human glioma cell line U118MG(Second report)." Neuroimmynological Rec.6. 205-209 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Choi B.O.: "Biochemical analysis of cytokine effect on the glycosphingolipid expression of human glioma cell lines." Tumor Res. 28. 9-18 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamaki T.: "The requirement of host immune response to tumor cells to achieve tumor eradication by intratumoral LAK cell infusion immunotherapy." Oncol.(Life Sci.Adv.). 13. 59-68 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maeda Y.: "Analysis of glicosphingolipid protein of human glioma cell lines and of reactivities of anti-GM3 and GM2 monoclonal antibodies." Neuroimmunological Rec.7. 89-93 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] B.O.Choi.: "Interleukin 4 enhances ganglioside GD3 expression on the human fibroblast cell line WI-38" J.Biochem.117. 315-320 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 八巻稔明: "サイトカインによるヒトグリオーマ細胞U118MGの糖脂質発現に及ぼす影響の解析 (第2報)" 神経免疫研究. 6. 205-209 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 崔炳旭: "ヒトグリオーマ細胞T98Gの細胞周期にかかわる糖脂質組成の解析" 神経免疫研究. 6. 200-204 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] B.O.Choi: "Biochemical analysis of cytokine effect on the glycosphingolipid expression of human glioma cell lines" Tumor Res.28. 9-18 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Yamaki: "The requirement of host immune response to tumor cells to achieve tumor eradication by intratumoral LAK cell infusion immunotherapy" Oncol.(Life Sci.Adv.). 13. 59-68 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 前田義裕: "ヒトグリオーマ細胞株の糖脂質分析と抗GM3およびGM2抗体の反応性の解析" 神経免疫研究. 7. 89-93 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] B.O.Choi: "Interleukin 4 enhances ganglioside GD3 expression on the human fibroblast cell line WI-38" J.Biochem.117. 315-320 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 八巻稔明: "サイトカインによるヒトグリオーマ細胞U118MGの糖脂質発現に及ぼす影響の解析(第2報)" 神経免疫研究. 6(印刷中). (1933)

    • Related Report
      1993 Annual Research Report
  • [Publications] 崔炳旭: "ヒトグリオーマ細胞T98Gの細胞周期にかかわる糖脂質組成の解析" 神経免疫研究. 6(印刷中). (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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