Role of Endothelin on Ischemic-reperfusion Injury of Limb
| Project/Area Number |
05807139
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | FUKUOKA UNIVERSITY |
|---|
Principal Investigator |
OJIMI Hiroyuki Fukuoka Univ.Fac.Medicine Assistant Prof., 医学部, 講師 (60160488)
|
|---|
| Project Period (FY) |
1993 – 1994
|
| Project Status |
Completed (Fiscal Year 1994)
|
| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | Endothelin / Ischemia / Reperfusion injury / Non-retlow plunomenon / 虚血・再灌流障害 / プロスタグラッディン / 虚血、再環流障害 |
|---|
| Research Abstract |
We measured the blood flow on the hind limb of rabbits using a laser doppler flow meter and/or a hydrogen clearance method. After ischemia for a duration of 8 hours, the blood flow of the hind limbs was decreased remarkably, compared with the 4 and 6 hour ischemia groups. the tissue of the skin and muscle was sharvested from experimetal limbs at preischemia, during ischemia, immediately after reperfusion, 15 and 30 minutes after reperfusion, and 1 and 2 hour after reperfusion. The EIA method was used to determine concentrations of endothelin and thromboxane A2 in the tissue. Concentrations of tissue endothelin and thromboxane A2 were directly proportional to the ischemic duration of the limb.
|
Report
(3 results)
Research Products
(10 results)
