| Project/Area Number |
05807152
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
ENDO Chikara Fukushima Medical University, School of Medicine, OB/GYN Lecture, 医学部・産婦人科, 講師 (50168829)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Uterus / IP_3 / Ca^<2+> / Oxytocin / LPS / Pregnancy / Endotoxin / 妊娠 / ラット |
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| Research Abstract |
<AIM> It is suggested that there is a strong association between systemic and intrauterine infection and preterm labor. The mechanisms responsible for the initiation of parturition under these circumstances have still not been completely established. In this study, pregnant Wistar rats on day 17 of gestation were sacrificed 3 hours after intraperitoneal injection of lipopolysaccharide (LPS) or vehicle. The effects of LPS was studied on IP_3 production, intracellular Ca^<2+> level and spontaneous contractions by oxytocin (OXT) of isolated uterine strips from preterm pregnant rats.
<RESULTS> 1. OXT significantly increased IP_3 production in both vehicle and LPS-treated rat uteri. The IP_3 production was significantly increased approximately 2 times by LPS treatment compared to control.
2. (1) OXT stimulated uterine contraction and intracellular Ca^<2+> level in pregnant rat uterus. However, in Ca^<2+>-free medium, OXT increased uterine contraction but not intracellular Ca^<2+> level. (2) OXT stimulated uterine contraction and intracellular Ca^<2+> level in LPS-treated pregnant rat uterus.
<CONCLUSIONS> 1. OXT increases IP_3 production in pregnant rat uterus. It was suggested that the mechanism of uterine contraction by OXT might be involved in Ca^<2+>-independent contraction. 2. LPS enhances IP_3 production by OXT in pregnant rat uterus. However, it is still unknown that LPS also enhances increasing intracellular Ca^<2+> level by OXT.
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