A study of liver cryopreservation

• Project/Area Number: 05807168
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 小児外科
• Research Institution: Nagoya City University
• Principal Investigator: MASHITA Keiji Nagoya City University Medical School, assistant, 医学部, 助手 (10181637)
• Co-Investigator(Kenkyū-buntansha): SHIRAI Tomoyuki Nagoya City University Medical School, professor, 医学部, 教授 (60080066) ; HASHIMOTO Takashi Nagoya City University Medical School, assistant professor, 医学部, 助教授 (10094393)
• Project Period (FY): 1993 – 1994
• Project Status: Completed (Fiscal Year 1994)
• Budget Amount: ¥1,800,000 (Direct Cost: ¥1,800,000); Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: liver preservation / bile production / liver perfusion / cryopreservation / 肝灌流 / 胆汁酸

Research Abstract

An ex vivo isolated perfused rat liver model was tested to assess viability of preserved liver.
Rats were pretreated with or without papaverine hydrochloride. Livers were taken out and perfused with TOM-2H organ preservation solution (made by Dr.IWANAKA,Tokyo University) and stored 4ﾟC before reperfusion with Krebs Ringel Bicarbonate Solution at 37ﾟC in a noncirculating perfusion system for l hr. From 30 mins after reperfusion, Taurocholate added into perfusion solution and examined bile flow, bile salt output, lactate dehydrogenase level every 5 mins during perfusion. Papaverine hydrochloride-pretreated livers produced significantly more bile flow and bile salt output than control liver s and also released significantly less lactate dehydrogenase level into the perfusate after reperfusion. Pathological finding shows destroyed ZONE 3 (pericentral area) in control livers, but it was not seen in papaverine hydrochloride-pretreated livers.
We supposed papaverine hydrochloride prevent vasoconstriction during preservation.