| Project/Area Number |
05807171
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | IWATE MEDICAL UNIVERSITY |
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Principal Investigator |
KANEKO Masaru Iwate Medical University DENTISTRY PROFESSOR, 歯学部, 教授 (60048270)
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| Co-Investigator(Kenkyū-buntansha) |
OHARA-NEMOTO Yuko Iwate Medical University DENTISTRY ASSISTANT, 歯学部, 助手 (10164667)
SASAKI Minoru Iwate Medical University DENTISTRY LECTURER, 歯学部, 講師 (40187133)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Streptococcus pyogenes / Streptococcus anginosus / Superantigen / Cytokine / Mitogenic factor / Periodontitis / Streptococcal pyrogenic exotoxin / Streptococcal pyrogenic exotoxi |
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| Research Abstract |
The relationship between biological activities of streptococcal pyrogenic exotoxins (SPE) produced by Streptococcus pyogenes and periodontitis, between biological activities of mitogenic factor (MF) from oral streptococci and periodonttitis were studied. SPEs were purified, and ELISA for detection of these toxins was achieved. These SPEs proliferated to human periferal blood mononuclear cells (PBMC), and promoted to growth T cell bearing TCR Vbeta2. TNF activity and IL-2 production were detected in culture supernatant of PBMC treated with SPEs, and induction of mRNA expression of TNF-alpha, TNF-beta, IL-2 and IFNgamma was observed in PBMC.Furthermore, We detected MF from culture supernatant of Streptococcus anginosus, and partially purified the factor. The MF proliferated to human PBMC,induced production of IL-2 and TNF,and expression of the cytokines mRNA.These results suggest that SPEs or MF from S.pyogens or S.anginosus isolated from oral cavity can be related to periodontitis.
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