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Identification of the factor responsible for the exhibition of behavioral abnormalities and the inner ear defects in mutant bustling mice (BUS/Idr).

Research Project

Project/Area Number 05808072
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionInstitute for Developmental Research, Aichi Human Service Center

Principal Investigator

YONEZAWA Satoshi ph.d.  Institute for Developmental Research, Aichi Human Service Center.Department of Embryology.Researcher., 発生学部, 室長 (90001867)

Project Period (FY) 1992 – 1994
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1993: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsmutant mouse / abnormal behavior / inner ear defects / c-mos expression / auditory brain-stem response / monoclonal antibody / c-mos発現 / 内耳平衡器破壊マウス / モデル動物 / プロトがん遺伝子 / c-mos / 内耳発生
Research Abstract

To identify the factor responsible for the exhibition of behavioral abnormalities of BUS mice, the inner ear and the CNS of the mutant mice were examined morphologically, biochemically and molecular biologically. TEM and SEM observations showed stereociliary derangements in the sensory hair cells of the cochlear Corti's organ and picnotic features of the vestibular sensory cells in the inner ear of BUS homozygotes (bus/bus), but not heterozygotes (<plus-minus>/bus). Audiological tests revealed that BUS homozygotes were deaf in their total life. Spongiform degeneration was observed in the CNS of BUS mice, but this lesion was found from outcross and intercross experiments to be due to a gene independent of bus. A feature of hemorrhage was often found in different parts of the brain stem in BUS homozygotes at later fetal stages, but some heterozygous fetuses also had dead cells clusters in the CNS,which indicated that the hemorrage in the fetal CNS could not be accepted as a cause of abno … More rmal bustling hehaviors in homozygotes. Since it has been reported that Mos transgenic mice displayd behavioral abnormalities and had the inner ear defects and the spongiform degeneration in the CNS,we tested a possibility of over-expression of c-mos in BUS homozygotes by means of the ribonuclease protection assay method. The results indicated, however, that the gene was normally expreessed in the CNS of homozygotes from later fetal stages to adult stages. Thus, we had no data providing a clue in the CNS for identifying the responsible factor for BUS abnormalities at the molecular level. In views of a possible functional association of the inner ear organs with behaviors in mice, the behaviors of chemically labyrinthectomized mice were examined compared with those of BUS homozygotes. As a result, both were found very similar in behaviors, suggesting that the inner ear defects, even if no lesions exist in the CNS,elicit abnormal behaviors in mice, including circling, head shaking, and hyperkinesia. We have developed in this study three hybridoma lines, each secreting an antibody recognizing specific cells in the inner ear organs : Y10F2 recognizing the vestibular supporting cells ; H3E11 recognizing the supporting cells of the macullae and the basal supporting cells of the cristae : and Y7E3 recognizing the supporting cells of the Corti's organ. Among these, monoclonal antibody Y10F2 was found to be useful for seeking the factor responsible for BUS abnormalities, providing the data that the immunoreactivity of cells of the inner ear organs and Y10F2 (stainability) was different between homozygotes and heterozygotes, at later fetal stages. Finally, efforts are being made to identify the responsible gene, bus. For this, several species of transcripts have been so far identified as quantitatively different between the inner ear of homozygotes and that of heterozygotes, as revealed by the method of mRNA differential displa Less

Report

(3 results)
  • 1995 Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Moriyama,K.et al: "Deutrium oxide's effect on defective vestibulocochlear system in mutant bustling(BUS)mice." Eguilib.Res.10. 31-36 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yonezawa,S.et al: "Cochlear pathology of the bustling mutant mouse,BUS/Idr." Acta Otolaryngol.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Otani,H.et al: "Vestibulocochlear defects and effects of deutrium oxide in mutant bustling(BUS)mice." Acta Otolaryngol.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Moriyama, K., Otani, H., Tanaka, O., Shoji, R., and Yonezawa, S.: "Deutrium oxide's effect on defective vestibulocochlear system in mutant bustling (BUS) mice." Equilib.Res.Suppl.0. 31-36 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yonezawa, S., Nodasaka, Y., Kamada, T., Fujita, S., Kato, K., Yamada, Y., Ogasawara, N., and Shoji, R.: "Cochlear pathology of the bustling mutant mouse, BUS/Idr." Acta Otolaryngol.(in press). (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Otani, H., Moriyama, K., Shoji, R., Yonezawa, S., and Tanaka, O.: "Vestibulocochlear defects and effects of deutrium oxide in mutant bustling (BUS) mice." Acta Otolaryngol.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Moriyama,K. et al.: "Deutrium oxide's sttect on defective vestibulocochlear system in mutant bustling (BUS)mice." Equi libRes.10. 31-36 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yonezawa,S.et al: "Cochlear pathology of the bustling mutant mouse,BUS/Idr." Acta Otolaryngol.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Otani,Moriyama,K.,Yonezawa,S.,Shoji,R.,Tanaka,O.: "Vestibulocohlear defects and ettects of deutrium oxide in mutant bustling(BUS)mice." Acta Otolaryngologica. (in press).

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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