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Cytological analysis of and strategy for retarded nerve regeneration in aging animals

Research Project

Project/Area Number 05834012
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 老化(加齢)
Research InstitutionYOKOHAMA CITY UINVERSITY

Principal Investigator

KOMIYAMA Atsushi  Yokohama City Uinversity School of Medicine, Department of Neurology, Assistant Professor, 医学部, 講師 (00170382)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1993: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsaging animals / nerve regeneration / Schwann cells / wallerian degeneration / cell proliferation / macrophages / 神経再生 / 細胞培養
Research Abstract

Although regeneration of myelinated fibers after nerve-crush was shown to be retarded in aging animals, the factors of this retardation remains to be determined. To test the hypothesis that Schwann cell dysfunction with aging process was at least in part responsible for the retarded response, we investigated proliferative responses of Schwann cells in the mouse sciatic nerves after nerve-transection at 2 and 24-29 months of age. As assessed by thymidine incorporation for 24h in culture (Komiyama and Suzuki, 1992a, 1994), Schwann cells from young adult nerves proliferated rapidly within day 1 post-transection and reached a peak at day 3. However, division rate of Schwann cells from aging mouse remained at the level about one tenth of Schwann cells from young adult mouse. The number of macrophages recruited in the distal stump was also smaller in the aging nerves than in the young adult nerves. When Schwann cells from the sciatic nerves of aging mouse were maintained in the media supplemented with 15% FBS,the rate of proliferation reached one half of that of young adult mouse during an 8 day period in culture. The results of our study suggest that Schwann cell proliferation is impaired in the injured nerves of aging mouse partly because of their limited capacity to proliferate. This difference in vivo and in vitro proliferative capacity of Schwann cells may be related to the paucity of macrophage migration in the endoneural space of injured nerves of aging mice.

Report

(2 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Ohno M: "Proliteration of microglia/macrophages in the demelinating…" Brain Res. 602. 268-274 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kano I: "Characterization of macrophages lineage colony stimulating tactor produced by thymic cells." Immunology. 79. 103-106 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohno M: "MHC Class II antigen expression and T-cell intiltration in the demyelinating CNS and PNS of the twitcher mouse" Brain Res. 625. 186-196 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komiyama A: "Progressive dystwnction of Twitcher Schwmon Cells is better eualuted in vitro than in vivo" Brain Res. 637. 106-113 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komiyama A: "Deprlesed rate of Schwann cell prolitention in aging induce during Wallerian degemeration" Brain Pathol. 4. 571- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamada H: "Schwann cell responces to foskolin and cyclic AMP analegens" Brain Res. 681. 97-104 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohno M,Komiyama A,Martin P,Suzuki K: "Proliferation of microglia/macrophages in the demyelinating CNS and PNS of twitcher mouse" Brain Res. 602. 268-274 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kamo I,Kunishita T,Kikuchi A,Nonaka I,Komiyama A: "Characterization of macrophage lineage colony stimulating factor produced by thymic myoid cells" Immunology. 79. 103-106 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komiyama A,Suzuki K,Hasegawa O,Kuroiwa Y: "Schwann cell dysfunction by exogenous psychosine in vitro and demyelination of the PNS in murine globoid cell leukodystrophy" Procceedings of the World Congress of Peripheral Nerve Toxicity (ed by Hashimoto), Tanaka Shobunndo, Niigata. 103-105 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohno M,Komiyama A,Martin P,Suzuki K: "MHC class ll antigen expression and T-cell infiltration in the demyelinating CNS and PNS of the twitcher mouse" Brain Res. 625. 186-196 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komiyama A,Suzuki K,Hasegawa O,kuroiwa Y: "Schwann cell proliferation is progressively impaired in murine globoid cell leukodystrophy" Can J Neurol Sci. 20. 76 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komiyama A,Suzuki K: "Progressive dysfunction of twicther Schwann cells is better evaluated in vitro than in vivo" Brain Res. 637. 106-113 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komiyama A,Suzuki K,Horie H,Hasegawa O,Kubota A: "Depressed rate of Schwann cell proliferation in aging mouse during Wallerian degeneration" Brain Pathol. 4. 571 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komiyama A,Suzuki K,Hasegawa O,Kubota A: "Relevance of impaired Schwann cell proliferation by exogenous psychosine to PNS demyelination to PNS demyelination in murine globoid cell keukodystrophy" Brain Pathol. 4. 458 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Komiyama A,Kondo K,Hasegawa O,Nakamura S,Ohno S: "Optic neuritis in cerebral toxocariasis" J Neurol Neurosurg Psychiatry. 59. 197-198 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamada H,Komiyama A,Suzuki K: "Schwann cell responses to forskolin and cyclic AMP analogues : comparative study of mouse and rat Schwann cells" Brain Res. 681. 97-104 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohno M: "Proliferation of microglia/macrophages in the demyelinating PNSECNS of tuitcher mouse" Brain Reseaich. 602. 268-274 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kamo I: "Charactenzation of macrophage lineage colony stimulatingfactor" Immunology. 79. 103-106 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Komiyama A: "Progressive dystunction of twitcher schwann cells is better evaluated in vitro than in vivo" Brain Research. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohno M: "MHL class II antiger expression and T-cell intiltration in the dewyelinoting" Brain Research. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Komiyama A: "Peripheral nerve toxicity" Tanaka Shobundo(in press), (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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