| Project/Area Number |
05835002
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
文化財科学
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| Research Institution | KYUSHU UNIVERSITY (1994-1995)
Saitama University (1993) |
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Principal Investigator |
KOIKE Hiroko Kyushu University, Graduate School of Social and Cultural Studies, Professor, 大学院比較社会文化研究科, 教授 (40107462)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Yoshiyuki Kyushu University, Graduate School of Social and Cultural Studies, Professor, 大学院比較社会文化研究科, 教授 (50128047)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | mtDNA / Human skeletons |
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| Research Abstract |
DNA amplification by PCR (Polymerase Chain Reaction) method and DNA sequencing provided great progress for the polymorphic analysis based on DNA sequences.
Mitochondrial DNA (mtDNA) has a rapid substitution rate compared with nuclear DNA,especially non-coding region in mtDNA is useful to the analysis of kinship relations among the same species. In this study, control region of mtDNA was analyzed to establish an experimental methodology using buried human skeletons.
(1) Materials for this studies should be in good preservation condition with relatively younger ages.
(2) Extraction of DNA : preparation of human skeletons under low temperature with liquid nitrogen, decalcification method with EDTA or HC1, and DNA extraction with Iso-Quick Kit were recommended for DNA extraction using human sekeletons.
(3) PCR method : Extracted DNA from human skeletons were relatively fragmented. Specific primers were designed to produce shorter PCR products.
(4)Sequencing Methods : PCR products need to be purified by microspin column. Cycle sequence method enabled DNA sequencing even using small amount of PCR products.
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