• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Angiogenesis in hepatocellular carcinoma

Research Project

Project/Area Number 05837003
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 血管生物学
Research InstitutionUniversity of Tokyo

Principal Investigator

OHNISHI Shin  University of Tokyo, Third Department of Internal Medicine, Clinical Associate., 医学部・附属病院, 助手 (00183236)

Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1993: ¥600,000 (Direct Cost: ¥600,000)
Keywordsvascular endothelial growth factor (VEGF) / angiogenesis / hepatocellular carcinoma (HCC)
Research Abstract

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), stimulates the growth of endothelial cells in vitro and promote angiogenesis in vivo. It has been proposed to be the most potent factor of tumor associated angiogenesis. VEGF is known to interact at least two specific high affinity receptor in tyrosine kinase family, Flt-1 and KDR/Flk-1. Since hepatocellular carcinoma (HCC) is one of tumors that are caracterized by marked angiogenesis, possible involvement of vascular endothelial growth factor expression in HCC cells is suspected. In this study, I investigated whether gene and protein expression of VEGF was increased in HCC cells. Northern blot hybridization analysis revealed that HCC cells exhibited a significant elevated level of VEGF mRNA.Immunohistochemical study also showed that intense immunoreactivity against VEGF on HCC tissues. In addition, VEGF secretion by HCC cells was confirmed by Western immunoblot study. No expression of flt-1 mRNA was detected in HCC cells. These findings support for the hypothesis that VEGF may be potent factor for angiogenesis associateted with HCC and may be strongly involved in the development and progression of the tumor.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Mashahiko Kuroda,Shin OHNISHI et al.: "Colocalization of Vascular Endothelial Growth Factor and Insulin in Pancreatic Islet Cells" Journal of Clinical Endocrinology and Metabolism. 80. 3196-3200 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroto Kita,Shin OHNISHI et al.: "HLA B44-restricted cytotoxic T lymphocyte responses to the peptides of HCV nucleoprotein residues 81-100 in patients with chronic hepatitis C" Journal of Gastroenterology. 30. 809-812 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroto Kita,Shin OHNISHI et al.: "A Helper T-Cell Antigen Enhances Generation of Hepatitis C Virus-Specific Cytotoxic T Lymphocytes in Vitro" Journal of Medical Virology. 45. 368-391 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuyo Yoshimi,Shin OHNISHI et al.: "Conparison of Hepatectony and Transcatheter arterial chenoenbolization for the treatment of Hepatocellular carcinoma" Hepatology. 16. 702-706 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshiaki GUNJI,Shin OHNISHI et al.: "Treatment of Hepatocellular Carcinoma associated with advanced cirrhosis by transcatheter arterial chenoenbolization using antologons blood clot" Hepatology. 15. 252-257 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Junji Shiga,Shin OHNISHI et al.: "Development and Growth Pattern of Small Hepatocellular Carcinomas in Woodchucks" Exp.Anim.40. 545-548 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masahiko Kuroda, Shin Ohnishi et al.: "Colocalization of Vascular Endothelial Growth Factor and Insulin in Pancreatic Islet cells." Journal of Clinical Endocrinology and Metablism. 80. 3196-3200 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroto Kita, Shin Ohnishi et al.: "HLA B44-restricted cytotoxic T lymphocyte response to the peptides of HCV nucleoprotein residues 81-100 in peptides with chronic active hepatitis C." Journal of Gastroenterology. 30. 809-812 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hiroto Kita, Shin Ohnishi et al.: "A Helper T-cell Antigen Enhances Generation of Hepatitis C Virus-specific Cytotoxic T Lymphocytes in vitro." Journal of Medical Virology. 45. 368-391 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fuyo Yoshimi, Shin Ohnishi et al.: "Comparison of Hepatectomy and Transcatheter Arterial Chemoembolization for the Treatment of Hepatocellular Carcinoma." Hepatology. 16. 702-706 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshiaki Gunji, Shin Ohnishi et al.: "Treatment of Hepatocellular Carcinoma Associated with Advanced Cirrhosis by Transcatheter Arterial Chemoembolization Using Autologous Blood Clot." Hepatology. 15. 252-257 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Junji Shiga, Shin Ohnishi et al.: "Development and Growth Pattern of Small Hepatocellular Carcinoma in Woodchuck." Experimental Animals. 40. 545-548 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masahiko Kuroda,Shin Ohnishi et al.: "Colocalization of Vascular Endothelial Growth Factor and Insulin in Pancreatic Islet Cells." Journal of Clinical Endocrinology and Metabolism. 80. 3196-3200 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroto Kita,Shin Ohnishi et al.: "HLA B44-restricted cytotoxic T lympkocyte responses to the peptides of HCV nucleoprotein residues 81-100 in patients with chromic hepatitis C" Journal of Gastroenterology. 30. 809-812 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroto Kita,Shin Ohnishi et al.: "A helper T-cell Antigen.enhances generation of Hepatitis C" Journal of Medical Virology. 45. 368-391 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fuyo Yoshimi,Shin Ohnishi et al.: "Comparison of Hepafectomy and Transcatheter arferial chemoembolization for the treatment of Hepatocellular Carcinoma" Hepatology. 16. 702-706 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toshiaki Gunji,Shin Ohnishi et al.: "Treatment of Hepatocellular Carcinema associated with advanced cirrhosis by transcathefer arterial chemoembolization using antologous blood clot" Hepatology. 15. 252-257 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] Junji Shiga,Shin Ohnishi et al.: "Development and Growth Pattern of Small Hepatocellular Carcinomas in Woodchucks" EXP.Anim.40. 545-548 (1991)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toshiaki GUNJI,Shin OHNISHI et al.: "Treatment of Hepatocellular Carcimoma associated with advanced cirrhosis by transcatheter arterial chemoembolization using antalogons blood lot" Hepatology. 15. 252-257 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fuyo Yoshimi,SHIN OHNISHI et al.: "Comparison of Hepatectomy and Transcatheter arterial chemoembolization for the treatment of Hepatocellular Carcinoma" Hepatology. 16. 702-706 (1992)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masahiko Kuroda,Shin Ohnishi et al.: "Co-lucalization of VEGF and Insulin in Pancreatic Zslet cells" Journal of Clinical Endocrinalogy and Metabolism. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Junji SHIGA Shin OHNISHI et al: "Development and Growth Pattern of Small Hepatocellular Carcinomas in Woodchucks" Exp.Anim.40. 545-548 (1991)

    • Related Report
      1993 Annual Research Report
  • [Publications] Toshiaki GUNJI Shin OHNISHI et al: "Treatment of Hepatocellular Carcinoma associated with advanced Cirrhosis by transcatheter arterial chemcembolization using autologous blood clot" Hepatology. 15. 252-257 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fuyo Yoshimi Shin OHNISHI et al: "Comparison of Hepatectomy and Transcatheter arterial Chemoembolization for the treatment of Hepatocellular Caranoma" Hepatology. 16. 702-706 (1992)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi