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Molecular approaches to the pathogenesis of glomerulanephritis by vasoactive substances

Research Project

Project/Area Number 05837007
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 血管生物学
Research InstitutionTokyo Medical & Dental University

Principal Investigator

TERADA Yoshio  Tokyo Medical & Dental University, Instructor, 医学部, 助手 (30251531)

Co-Investigator(Kenkyū-buntansha) TOJO Naoko  Tokyo Medical & Dental University, Instructor, 医学部, 助手 (90227554)
TOMITA Kimio  Kumamoto Univ., Medical School, Professor, 医学部, 教授 (40114772)
MARUMO Fumiaki  Tokyo Medical & Dental University, Professor, 医学部, 教授 (00050443)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥800,000 (Direct Cost: ¥800,000)
Keywordsgene expression / PCR / cell proliferation / glomerulonephritis / PDGF / CNP / MAP kinase / signal transduction / エンドセリン / 抗利尿ホルモン
Research Abstract

Renal nephron segments were heterogeneous, and receptors for endothelin (ET)-1, ET-3, Angiotensin II (AT II), EGF,and IGF-1 distribute differently along the nephron segments. We have revealed that the PCR localizatoin of two types (V2, V1) of vasopressin receptor, and angiotensin II receptor. We also reported that localization of mRNA and protein of ET-3 along the nephron segments. Recently, growth factors and vasoactive substances are reported to stimulate mitogen-activated protein kinase (MAP-K). In this study, we showed that mRNA of MEK-K,Raf-1-K,MAPK-K,MAP-Ks(p42 and p44), and S6-K was expressed in nephron segments using RT-PCR technique. We showed that MAP-K cascades are expressed in every nephron segment. ET-1, ET-3, AT II,EGF,and IGF-I stimulate MAP-K cascades heterogeneously along the nephron segment. We concluded that MAP-K cascades play an important role in the regulation of renal function. We also reported that the presence of C-type natriuretic factor and its receptor (GC-B) along the nephron. The CNP system may play an important roles in modulatiing the salt and body fluid homeostasis and blood pressure. We also analyzed the expression of PDGF and PDGF-receptor expression in the glomeruli of IgA nephropathy patients. Thus, we investigated the new approaches to the pathogenesis of glomerulonephritis and renal functions.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Yoshio.Terada et al: "Different lodalization and regulation of two types of vasopressin recepter messenger RNA in microdissected rat nephron segments using RT-PCR" Jourral of clinical Investigation. 92. 2339-2345 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshio Terada et al: "PCR localization of argiotensin II receptor and angiotensinogen mRNAs in rat kidney" Kidney International. 43. 1251-1259 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshio Terada et al: "Expression of endothelin-3 mRNA along rat nephron segmerts using polymerase chain reaction" Kidney International. 44. 1273-1280 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tianxin Yang et al: "Effect of hyperosmolality on production and mRNA expression of ET-1 in inner medullary collecting duct." American Jourral of physiology. 264. F684-F689 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshio Terada et al: "Seguential Activation of raf-1-kinase,MAP-kinase-kinase,MAP-kinase and S6kinase by hyperosmolality in renal cell" Journal of Biological chemistory. 269. 31296-31301 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshio Terada et al: "PCR localization of C-type natricvetic peptide and B-type receptor mRNAs in rat nephron segments" American Journal of Physiology. 267. F215-F222 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Terada, K.Tomita, H.Nonoguchi, F.Marumo: "PCR localization of angiotensin II receptor and angiotensnogen mRNAs in rat kidney." Kidney International. 43. 1251-125

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Terada, K.Tomita, H.Nonoguchi, T.Yang, F.Marumo: "Different localization and regulation of two types of vasopressin receptor messenger RNA in microdissected rat nephron segments using reverse transcription polymerase chain reaction." Journal of Clinical Investigation. 92. 2339-2345 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Terada, K.Tomita, H.Nonoguchi, T.Yang, F.Marumo: "Expression of endothelin-along rat nephron segments using PCR." Kidney International. 344. 1273-1280 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yang, Y.Terada, H.Nonoguchi, K.Ujiie, K.Tomita, F.Marumo: "Effect of hyperosmolality on production an mRNA expression of ET-1 in inner medullary collecting duct." American Journal of Physiology. 264. F684-F689 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Terada, K.Tomita, M.K.Homma, H.Nonoguchi, T.Tang, T.Yamada, Y.Yuasa, E.G.Krebs, F.Marumo: "Sequential activation of raf-1 kinase, MAP kinase kinase, MAP kinase, and S6 kinase by hyperosmolality in renal cell." Journal of Biological Chemistry. 269. 31296-31301 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Terada, K.Tomita, H.Nonoguchi, T.Yang, F.Marumo: "PCR localization of C-type natriuretic peptide and B-type receptor mRNAs in rat nephron segments." American Journal of Physiology. 267. F215-F222 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Otuka, Y.Terada, T.Yang, H.Nonoguchi, K.Tomita, F.Marumo: "Localization of cyclophilin A and cyclophilin C mRNA in murine kidney using RT-PCR." Kidney International. 45. 1340-1345 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yang, Y.Terada, H.Nonoguchi, M.Tsujino, K.Tomita, F.Marumo: "Kallikrein-binding protein mRNA expression in the kidney : localization along the nephron and difference between SHR and WKY rats." American Journal of Physiology. 267. F325-F330 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Owada, K.Tomita, Y.Terada, H.Sakamoto, H.Nonoguchi, F.Marumo: "Endothelin-stimulates cGMP production via ETB receptor by producing nitric oxide in isolated rat glomerulus, and in cultured rat mesangial cells." Journal of Clinical Investigation. 393. 556-563 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yoshio Terada: "Sequential Activation of raf-1 kinase,MAP-kinase,Kinase,MAP kinase and s6 kinase by hyperosmolality in vevol cell" Journal of Biological Chemistory. 269. 31296-31301 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshio Terada: "PCR localization of C-type natviuretic peptide and B-type vcceptor mRNAs in rat nephron segment." American Journal of Physiology. 267. F215-F222 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masakazu Otuka: "Localization of cyclophilm A and cyclophilmC mRNA in murine Kidney using RT-PCR" Kidney International. 45. 1340-1345 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tianzin Yang: "Kallikrein-binding protein mRNA expression in the kidney,localization along the nephrin and diffevence between SHR and WKY rats" American Journal Physiology. 267. F325-F330 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Akira Owada: "Endothelin-3 stimulates cGMP production via ETB receptor by production nitruc oxide in isolated glomercluer and in culfuved rat mesaugicl cell" Journal of Clinical Investigation. 93. 556-563 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshio Terada: "Sequential Activation of MAP kinase cascad by Angutensn II in OK cells" Kidney Internatinal. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Yoshio Terada et al: "Different localization and regulation of two types of vasopressin recepter messenger RNA in microdissected rat nephron segments using RT-PCR" Journal of Clinical Investigation. 92. 2339-2345 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshio Terada et al: "PCR localization of angiotensin II receptor and angiotensinogen mRNAs in rat kidney" Kidney International. 43. 1251-1259 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshio Terada et al: "Expression of endothelin-3 mRNA along rat nephron segments using polymerase chain reaction" Kidney International. 44. 1273-1280 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tianyin Yang et al: "Effect of hyperosmolality on production and mRNA expression of ET-1 in inner medullary collecting cluct." American Journal of Physiology. 264. F684-F689 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kimio Tomita et al: "Effects of ET-1 on water and chloride transport in cortical collecting clucts of the rat" American Journal of Physiology. 264. F690-F696 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshio Terada et al: "PCR localization of C-type natricvetic peptide and B-type receptor mRNAs in rat nephron segments" American Journal of Physiology. (印刷中). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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