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Interactions of human blood vessel endothelial cells and smooth muscle cells

Research Project

Project/Area Number 05837027
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 血管生物学
Research InstitutionTokyo Metropolitan Institute of Gerontology (TMIG)

Principal Investigator

KAJI Kazuhiko  TMIG,Biochmistry Isotopes Research Scientist, アイソトープ部門, 主任研究員 (40073019)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1993: ¥900,000 (Direct Cost: ¥900,000)
KeywordsHuman endothelial cells / Human smooth muscle cells / FGF / Apoptosis / Paracrine / TGF-β / TGFβ / PDGF / 細胞増殖
Research Abstract

Surivival and proliferation of human blood vessel endothelial cells (HBVEC) are influenced by fibroblast growth factor (FGF). Removal of FGF from the medium of the cells in culture resulted in death of the cells. The death caused by deprivation of FGF is active death (apoptosis), and the process of apoptosis can be inhibited by cycloheximide. Other mitogenic agents, such asEGF,PDGF,TGFs, ILl, and transferrin can not stop the HBVEC apoptosis. The effect of FGF is mimicked by a phorbol ester and is prevented by an inhibitor of protein kinase C.This mechanism is though to be important for the regulation of vascular organization through the degeneation of vessels. As smooth muscle cells produce FGFs, it seems that HBVEC accect the FGFs and survive from the smooth muscle cells like a paracrine fashion in vivo conditions. Several substances also induce or modify apoptosis on HBVEC.Hemorrhagic snake venoms induce apoptotic cell death, but neurotoxic snake venoms do no induce programd cell dea … More th. No effect of hemorrhagci venom is observed with many types of cultured cells other than HBVEC.Hemorrhagic snake venom appears to be a useful tool for studies of molecular mechanisms of vascular apoptosis. Addition of the alkyllysophodpholipid ET16-OMe, a putative antitumor drug, to the culture of HBVEC results in apoptotic cell death. The mechanism responsible of apoptosis induced by this drug has unique characteristics, as compared to those of apoptosis induced by other antitumor drugs, since the drug caused fragmentation of dying cells and its effect can be overcome by the presence of a survival factor, namely, FGF.Nitric oxide synthase (NOS) activity is enhanced in HBVEC by the combined stimulation of IFN-_Y plus IL-1beta, gamma TNF-alpha and PLS.The enhanced NOS production is associated with the death of the cells. NOS inhibitors such as nitro-L-arginine enhance survival of HBVEC.DNA analysis of the NOS induced HBVEC shows that internucleosomal DNA fragmentation has occurred, suggesting that apoptosis is at least partially induced by the excess NO production. Less

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Araki, S.: "Induction of apoptosis by hemorrhagic snake venom in vasavlan endothelial cells" Biochem. Biophys. Res. Commun.190. 148-153 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, I.: "Augmentation of endothelin-1, prostacyctin and thromboxane A_2 secretion associated with in vitro ageing in cultured human umbilical vein endothelial cells" Mech. Age. Develop.71. 73-84 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, I.: "Reduotion of nitric oxide producing actirity associated with in vitro aging in cutared human umbilical vein endothelial cells" Biochem. Biophys. Res. Commun.195. 1070-1076 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kaneko, T.: "Protective effect of lipophyilic derivatives of ascorbic acid on lipid peroxide-induced endothelial injury" Arch. Biochem. Biophys.304. 176-180 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Araki, S.: "Programoned all death in response to alkyllsophospholipids in endothelial cells" J. Biochem.115. 245-247 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsuji, S.: "Decay-accelerating factor on human umbilical vein endothelial cells : Its histamine-induced expression and spontanevus rapid shedding from the cell surface" J. Immunul.152. 1404-1410 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Araki, S., Ishida, T., Yamamoto, T., Kaji, K.and Hayashi, H.: "Induction of apoptosis by hemorrhagic snake venom in vascular endothelial cells." Biochem.Biophys.Res.Commun.190. 148-153 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, I., Kaji, K., Morita, I., Nagao, M.and Murota, S.: "Augmentation of endothelin-1, prostacyclin and thromboxane A2 secretion associated with in vitro ageing in cultured human umbilical vein endothelial cells." Mech.Age.Develop.71. 73-84 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, I., Morita, I., Kaji, K., Ikeda, M.Nagao, M.and Murota, M.: "Reduction of nitric oxide producing activity associated with in vitro aging in cultured human umbilical vein endothelial cells." Biochem.Biophys.Res.Commun.195. 1070-1076 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kaneko, T., Kaji, K.and Matsuo, M.: "Protective effect of lipophyillic derivatives of ascorbic acid on lipid peroxide-induced endothelial injury" Arch.Biochem.Biophys.304. 176-180 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Araki, S., Tsuna, I., Kaji, K.and Hayashi, H.: "Programd cell death in response to alkylllsophospholipids in endothelial cells" J.Biochem.115. 245-247 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tsuji, S., Kaji, K.and Nagasawa, S.: "Decay-accelerating factor on human umbilical vein endothelial cells : Its histamine-induced expression and spontaneous rapid shedding from the cell surface." J.Immunol.152. 1404-1410 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, I., Kaji K.and Murota, S.: "Age related decline in cytokine induced apoptosis of cultued endothelial cells partially mediated by nitric oxide." Mech.Age.Develop.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Araki,S.Kaji,K.et al: "Programmed cell denth in response to alhyllsophospholipids in endethelial cells" J.Biochem.115. 245-247 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsuji.,S,Kaji,K.et al: "Decay-accelerating factor on human umbilical vein endothelial cells" J.Immunol.152. 1404-1410 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,I.,Kaji,K.et al: "Age related decline in cytohine induad apoptosis of cultaked endothelial cells partially mediated by niforic oxide" Mech.Age.Develop.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 加治和彦: "内皮細胞の増殖制御と老化" 日経サイエンス. 1994(9). 26-31 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,I.,Jaji,K.,et al.: "Augmentation of endothelin-I,prostacylin and thromboxane A_2 secretion associated with in vitro ageing in caltured human endothelial cells." Mech.Age. Develop.71. 73-84 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato,I.,Jaji,K.,et al.: "Reduction of nitric oxide producing actirity associated with in vitro aging in caltured human umbilical vein endothelial cells." Biochem.biophys.Res.Commun. 195. 1070-1076 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Araki,S.,Kaji.K.,et al.: "Programned cell death in response to alkyllysophaspholipids in endothelial cells." J.Biochem.115. 245-247 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tsuji,S.,Kaji,K.,et al.: "Decay-accelerating factor on human umbilical vein endothelial cells." J.Immanol.152. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 加治和彦,平井玲子: "血管内皮細胞をin vitroでエイジングさせる方法" 血管と内皮. 3. 493-502 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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